Specific primer pair for rapid identification of alien invasive Pomacea in China based on multiple PCR (Polymerase Chain Reaction) and application of specific primer pair

Abstract

The invention discloses a multiple specific primer pair for assisting in identification and detection of alien invasive species of Pomacea canaliculata and Pomacea maculata and further discloses application of the multiple specific primer pair, aiming to overcome the difficulty in identifying and distinguishing Pomacea canaliculata and Pomacea maculata according to morphological characteristics because Pomacea canaliculata and Pomacea maculata are relative species with similar morphology. The specific primer pair is composed of DNA sequences expressed by a sequence 1, a sequence 2 and a sequence 3 of a sequence table. The specific primer pair can be applied to help to identity the alien invasive species of Pomacea canaliculata and Pomacea maculata molecularly without being affected by individual growth states or sample integrity, and has the advantages of high economic efficiency, convenience, accuracy and high sensitivity. The specific primer pair and the application thereof can be applied to plant protection departments and quarantine department in a wide scope and solves the problem about identification of the alien invasive species of Pomacea canaliculata and Pomacea maculata in China, which troubles the plant protection and quarantine departments for a long time.

Description

technical field [0001] The invention relates to a group of specific primer pairs for assisting in the identification of alien invasion organisms apple snails and applications thereof, belonging to the field of biotechnology. Background technique [0002] The apple snail belongs to the mollusca (Mollusca), gastropod (Gastropoda), new gastropod (Caeogastropoda), bottle snail family (Ampullariidae), apple snail ( Pomacea Perry, 1810). The apple snail was introduced and colonized in my country as a food in the early 1980s, but it was abandoned by people due to its poor taste, and it was bred in large numbers in the field; at present, the snail has rapidly expanded to Zhejiang, Fujian, Guangdong, Guangxi, and Hainan , Yunnan, Guizhou, Jiangxi, Sichuan, Chongqing, Hunan and Anhui and other provinces and caused disasters. Apple snails can harm a variety of aquatic crops and cause serious losses to rice production in southern my country; at the same time, apple snails can destroy t...

AI Technical Summary

Problems solved by technology

small tube apple snail P. canaliculata with spotted apple snails P. maculata As closely related species, they are highly similar in many aspects such as morphological characteristics and living habits; in addition, due to the high environmental adaptability of apple snails, they are easily affected by factors such as food sources, external environments, and developmental stages, and are prone to intra-species morphological variation , so there are great difficulties and uncertainties in identifying the two species of apple snails morphologically.

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