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Method for cultivating black fungus strains by aid of decomposable pine and cypress sawdust

A cultivation method and black fungus technology, applied in the field of black fungus strains, can solve the waste of sawdust and other problems, and achieve the effects of avoiding waste, simple process and low cost

Active Publication Date: 2016-06-01
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the wood chips used in the cultivation of black fungus are all non-pine and cypress wood chips. At present, the wood chips of a large number of pine and cypress trees in my country can only be cultivated and utilized after years of accumulation and fermentation to remove the coniferous resin contained in the wood chips, resulting in a large number of waste of such sawdust

Method used

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  • Method for cultivating black fungus strains by aid of decomposable pine and cypress sawdust
  • Method for cultivating black fungus strains by aid of decomposable pine and cypress sawdust

Examples

Experimental program
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Effect test

Embodiment 1

[0018] (1) Take a little flesh or ejected spores of wild black fungus fruiting bodies growing on pine and cypress trees, place them on a pre-prepared PDA medium in an open environment, and then place the medium in a room at 25°C Cultivate for 7 days, until mycelia grow out;

[0019] (2) Transfer the hyphae obtained in step (1) to ordinary PDA medium (200 g of potatoes boiled in water for filtration, 20 g of glucose and 20 g of agar), and then place the culture medium in a room at 25° C. for 7 days. silk grows;

[0020] (3) The mycelium obtained in step (2) is transferred to the pre-prepared pine and cypress sawdust medium (pine and cypress sawdust 78wt%, bran 20wt%, sugar 1wt% and gypsum 1wt%) bacterial bag (35cm × 17cm) , then place the bacteria bag in a room at 25°C for 30 days, until the mycelium is overgrown;

[0021] (4) Transfer the hyphae obtained in step (3) from the pine and cypress wood chip fungus bag to ordinary PDA medium, and place it in a room at 25° C. for 7 ...

Embodiment 2

[0023] (1) Take a little flesh or ejected spores of wild black fungus fruiting bodies growing on pine and cypress trees, place them on a pre-prepared PDA medium in an open environment, and then place the medium in a room at 26°C Cultivate for 8 days, until mycelia grow out;

[0024] (2) Transfer the hyphae obtained in step (1) to ordinary PDA medium (200 g of potatoes boiled in water for filtration, 20 g of glucose and 20 g of agar), and then place the culture medium in a room at 25° C. for 7 days. silk grows;

[0025] (3) The mycelium obtained in step (2) is transferred to the pre-prepared pine and cypress sawdust medium (pine and cypress sawdust 78wt%, bran 20wt%, sugar 1wt% and gypsum 1wt%) bacterial bag (35cm × 17cm) , and then place the bacterial bag in a room at 26°C for 40 days until the hyphae are overgrown;

[0026] (4) Transfer the hyphae obtained in step (3) from the pine and cypress wood chip fungus bag to ordinary PDA medium, and place it in a room at 26° C. for...

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Abstract

The invention relates to a method for cultivating black fungus strains by the aid of decomposable pine and cypress sawdust. The method includes (1), collecting black fungi grown on pine trees, transferring the black fungi in culture media and cultivating the black fungi in the culture media in an isolated manner; (2), transferring the black fungi in ordinary culture media and cultivating the black fungi in the ordinary culture media; (3), inoculating the black fungi in fungus bags with culture media with the pine and cypress sawdust and cultivating strains until the fungus bags are filled with the grown strains; (4), transferring the strains from the fungus bags into ordinary PDA (potato dextrose agar) culture media. The method has the advantages that processes are simple, the cost is low, the pine and cypress sawdust can be sufficiently utilized for cultivating the black fungi as compared the cultivated black fungus strains to conventional black fungus strains, wastage of a large quantity of pine and cypress sawdust can be prevented, and the method for cultivating the black fungi in China is unique and has an excellent application prospect.

Description

technical field [0001] The invention belongs to the field of black fungus strains, in particular to a method for cultivating black fungus strains capable of decomposing pine and cypress wood chips. Background technique [0002] my country is a big country in the cultivation of edible fungi, especially the cultivation of black fungus is widespread in China, and black fungus is cultivated from south to north. However, the wood chips used in the cultivation of black fungus are all non-pine and cypress wood chips. At present, the wood chips of a large number of pine and cypress trees in my country can only be cultivated and utilized after years of accumulation and fermentation to remove the coniferous resin contained in the wood chips, resulting in a large number of Waste of such sawdust. Therefore how to cultivate the black fungus strain that can decompose and utilize pine and cypress sawdust is one of the key factors to solve this type of sawdust waste. Contents of the inven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 李传华陈明杰张劲松沈学香奚莉萍
Owner SHANGHAI ACAD OF AGRI SCI