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Bacillus subtilis biological preservative and application thereof to preservation of large yellow croakers

A technology of Bacillus subtilis and biological preservative, which is applied in the field of preservative preservation of aquatic products, can solve the problem of high price, and achieve the effects of inhibiting growth, wide antibacterial spectrum and maintaining freshness

Inactive Publication Date: 2016-06-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Animal / plant-derived preservatives are limited by raw materials, and the price is high; while the current industrialized microbial-sourced preservatives are microbial metabolites, which are increasingly valued

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] (3) Preparation of Bacillus subtilis aseptic fermentation broth: the fermentation broth prepared in step (2) is connected to the seed broth prepared in step (1), the inoculum is 7%; the initial pH value is adjusted to 8; Temperature 38℃, rotation speed 100r·min -1 , The filling volume is 150mL / 250mL shake flask; culture 24h under these conditions;

[0027] (4) Preparation of sterile fermentation broth: After centrifuging the Bacillus subtilis culture broth described in step (3) at 10,000 r / min for 15 minutes, it is filtered with a 0.22 μm filter membrane to obtain sterile fermentation filtrate;

[0028] (5) Preparation of crude fermentation broth: adjust the sterile fermentation broth described in step (4) to pH 2.0 with 2mol / L HCl, place it at 4°C for 3 days, centrifuge again at 10000r / min for 10min, and collect the precipitate. Dissolve in sterile water and adjust the pH to 7 with NaCl to obtain a crude extract of Bacillus subtilis;

[0029] The crude extract of Bacillus su...

Embodiment 1

[0031] A composite preservative of crude extract of Bacillus subtilis-Nisaprin-chitosan hydrochloride is used for preservation. Spray about 10mL of preservative evenly on the front and back of the chilled large yellow croaker.

[0032] Storage: Store large yellow croaker on ice at 4°C. After storage for 5 days (d), the total number of colonies exceeds 10 5 cfu / g, after 6 days (d), the volatile base nitrogen (TVB-N) content exceeds 25mg / 100g, and the shelf life is over.

Embodiment 2

[0034] The crude extract of Bacillus subtilis-chitosan hydrochloride compound antistaling agent is used for preservation. Spray about 10mL of preservative evenly on the front and back of the chilled large yellow croaker.

[0035] Storage: Store large yellow croaker on ice at 4°C. After storage for 3.5 days (d), the total number of colonies exceeds 10 5 cfu / g, 4 days (d) after the volatile base nitrogen (TVB-N) content exceeds 25mg / 100g, the shelf life ends.

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PUM

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Abstract

The invention provides application of bacillus subtilis to preservation of large yellow croakers as a biological preservative. The bacillus subtilis is bacillus subtilis BSh0851. The invention further provides a preservation method of the large yellow croakers; the fresh large yellow croakers are inactivated by crushed ice, and bacillus subtilis rough extract, nisaplin and a chitosan solution are mixed and then are sprayed on fish bodies; and then the large yellow croakers are stored on ice in a refrigerator at 4 DEG C. The method has a simple and safe process; the preservation and fresh-keeping effects are remarkable; and the shelf life of aquatic products is effectively prolonged, and original freshness, flavor and texture of the aquatic products can be kept very well.

Description

Technical field [0001] The invention belongs to the antiseptic and fresh-keeping technology of aquatic products, and specifically relates to the application of Bacillus subtilis as a biological antistaling agent in the antiseptic and fresh-keeping method of large yellow croaker and a fresh-keeping and antiseptic method. Background technique [0002] Aquatic products are an important part of people's food, and ice preservation is one of the main ways for aquatic products to circulate in the market. Because aquatic products are rich in protein and other nutrients, it is easy to cause spoilage bacteria to grow and multiply rapidly, and a large amount of spoilage material accumulates, so it is very easy to deteriorate, affects the quality of aquatic products, and even threatens human health. Therefore, the fresh-keeping method of aquatic products has become a current research hotspot. At present, the preservation technologies applied to aquatic products mainly include low-temperatur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B4/20A23B4/09
Inventor 张雯张绍升倪莉
Owner FUZHOU UNIV
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