Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of content determination method of malonyl ginsenoside

A technology for the determination of malonyl ginsenosides, which is applied in the field of content determination of malonyl ginsenosides by column switching high performance liquid chromatography, and can solve difficult methodological investigations, inability to determine the content of malonyl ginsenosides, and reference substances Can not be stored and transported for a long time to achieve the effect of high precision

Inactive Publication Date: 2017-04-12
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The disadvantage of this method is that, first, there are at least two kinds of malonyl ginsenosides that are hydrolyzed to produce the same neutral ginsenoside, and there may be many kinds, so the content of each malonyl ginsenoside cannot be determined; second, Due to the unstable chemical properties of malonyl ginsenoside, its reference substance cannot be stored and transported for a long time; third, this type of compound is stable in solution for less than 6 hours at room temperature, and it is difficult to complete routine tests with itself as a reference substance During the methodological investigation in the process, the reliability of the data could not be determined
And detection method in the prior art all can not solve above-mentioned technical defect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of content determination method of malonyl ginsenoside
  • A kind of content determination method of malonyl ginsenoside
  • A kind of content determination method of malonyl ginsenoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment one: adopting ginsenoside Rf as reference substance to measure the content of malonyl ginsenoside Rf in 8-year-old ginseng, the specific operations are:

[0030] ① Separating ginsenosides in sample A to be tested by chromatographic column C-1 to obtain component B containing malonyl ginsenosides;

[0031] ②Through valve switching, UV, P-2 and E-1 are connected, so that the eluted component of malonyl ginsenoside component B passes through the enrichment column E-1, and at the same time, the regulator is added through the pump P-2 to change the outflow group The composition of the solvent, so that the malonyl ginsenoside component B and other components are completely adsorbed on the enrichment column E-1;

[0032] ③ After the completion, switch the valve to connect the pump P-3 and the enrichment column E-1. At this time, the enrichment column E-2 is connected with the UV detector and P-2; the pump P-3 is connected with the enrichment column E-1 ; P-3 pumpin...

Embodiment 2

[0046] Embodiment two: using ginsenoside Rf as reference substance to measure the content of malonyl ginsenoside Rf in 8-year-old ginseng, the specific operations are:

[0047] ① Separating ginsenosides in sample A to be tested by chromatographic column C-1 to obtain component B containing malonyl ginsenosides;

[0048] ②Through valve switching, UV, P-2 and E-1 are connected, so that the eluted component of malonyl ginsenoside component B passes through the enrichment column E-1, and at the same time, the regulator is added through the pump P-2 to change the outflow group The composition of the solvent, so that the malonyl ginsenoside component B and other components are completely adsorbed on the enrichment column E-1;

[0049] ③ After the completion, switch the valve to connect the pump P-3 and the enrichment column E-1. At this time, the enrichment column E-2 is connected with the UV detector and P-2; the pump P-3 is connected with the enrichment column E-1 ; P-3 pumping t...

Embodiment 3

[0063] Embodiment three: using ginsenoside Rf as reference substance to measure the content of malonyl ginsenoside Rf in 8-year-old ginseng, the specific operations are:

[0064] ① Separating ginsenosides in sample A to be tested by chromatographic column C-1 to obtain component B containing malonyl ginsenosides;

[0065] ②Through valve switching, UV, P-2 and E-1 are connected, so that the eluted component of malonyl ginsenoside component B passes through the enrichment column E-1, and at the same time, the regulator is added through the pump P-2 to change the outflow group The composition of the solvent, so that the malonyl ginsenoside component B and other components are completely adsorbed on the enrichment column E-1;

[0066] ③ After the completion, switch the valve to connect the pump P-3 and the enrichment column E-1. At this time, the enrichment column E-2 is connected with the UV detector and P-2; the pump P-3 is connected with the enrichment column E-1 ; P-3 pumping...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed is a content measuring method of malonyl-ginsenoside. No reference substance is used, and accuracy in measuring is realized. The content measuring method includes: collecting components containing malonyl-ginsenoside in a to-be-measured sample from high performance liquid chromatography outflow components through vavle switching; hydrolyzing the components to obtain hydrolyzate; measuring content of corresponding neutral ginsenoside in the hydrolyzate, wherein the content is equal to amount of substance of malonyl-ginsenoside in the to-be-measured sample; converting to acquire content of malonyl-ginsenoside in the sample. The content measuring method is simple, quick and high in measuring result precision and accuracy and can be used for qualitative and quantitative analysis and quality control of malonyl-ginsenoside in the sample.

Description

technical field [0001] The invention belongs to the field of pharmaceutical analysis methods, and relates to a method for determining the content of malonyl ginsenosides, in particular to a method for determining the content of malonyl ginsenosides by column switching high performance liquid chromatography. Background technique [0002] The reference substance is the basic reference for measuring the value of chemical components in chemical quantitative analysis, and it is of great significance in pharmaceutical analysis. However, it is difficult to obtain sufficient reference substances for many chemical components, or their reference substances are difficult to preserve due to unstable physical and chemical properties. In this case, it is difficult to measure the exact amount of the chemical components. [0003] Malonyl ginsenoside (malonyl-ginsenoside) is called malonyl ginsenoside, and its content is very high in fresh ginseng and its medicinal materials. Among them, the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 肖盛元符洋洋
Owner BEIJING INSTITUTE OF TECHNOLOGYGY