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Drug composition of amantadine hydrochloride and medical application of drug composition

A technology of amantadine hydrochloride and its composition, which is applied in the field of biomedicine, can solve the problems that have not been reported related to amantadine hydrochloride, etc., and achieve the effects of highlighting substantive features, significant progress, and improved neuroprotective effect

Inactive Publication Date: 2016-06-08
WENZHOU JUYICHUANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there has been no report on the correlation between the pharmaceutical composition of amantadine hydrochloride and neuroprotection

Method used

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  • Drug composition of amantadine hydrochloride and medical application of drug composition
  • Drug composition of amantadine hydrochloride and medical application of drug composition
  • Drug composition of amantadine hydrochloride and medical application of drug composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0019] Sources of reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane were of analytical grade, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, of analytical grade, were purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.

[0020] Separation method: (a) Grind the dried leaves of Panax notoginseng (5kg), extract with 75% ethanol under heat reflux (20L×3 times), combine the extracts, concentrate until no alcohol smell (4L), and then use petroleum ether (4L ×3 times), ethyl acetate (4L×3 times) and water-saturated n-butanol (4L×3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) In step (a), the ethyl acetate extract was removed with D101 macroporous resin, first eluted with 8% ethanol for 6 column volumes, then 70% ethanol for 8 column volumes, and collected 70% eluate, C...

Embodiment 2

[0023] Example 2: Neuroprotection

[0024] 1. Materials and Instruments

[0025] The PC12 cell line was donated by the Experimental Center of Anhui University of Traditional Chinese Medicine. Aβ 1-40 Protein was purchased from Sigma Company. Compound (I) is self-made, and the HPLC normalized purity is greater than 98%. DMEM / F12 medium (imported packaging was purchased from Beijing Thermo Fisher Biochemical Products Co., Ltd. NewbomCalfSerum was purchased from Beijing SolarbioScience&Technology Co., Ltd. MTT was purchased from Amresco, USA. KGI AnnexinV-FITC Cell Apoptosis Detection Kit was purchased from From Nanjing KGI Biotechnology Co., Ltd. Paraformaldehyde was purchased from China Pharmaceutical Group Chemical Reagent Co., Ltd. Bcl-2 polyclonal antibody was purchased from Wuhan Boster Biotechnology Co., Ltd. Bax polyclonal antibody was purchased from Beijing Boaosen Biotechnology Co., Ltd. Cyt-c polyclonal antibody was purchased from Wuhan Boster Biotechnology Co., Lt...

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PUM

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Abstract

The invention discloses a drug composition of amantadine hydrochloride and medical application of the drug composition. The drug composition of the amantadine hydrochloride is prepared from the amantadine hydrochloride and a natural product compound (I) separated from the dry leaf of radix notoginseng and having a novel structure; when the amantadine hydrochloride and the natural product compound (I) are singly used, a neuroprotective effect is weaker; when the amantadine hydrochloride and the natural product compound (I) are combinedly used, the neuroprotective effect is remarkably improved. The drug composition can be developed into a neuroprotective drug, and has outstanding substantive distinguishing features and a remarkable progress in comparison to the prior art.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of amantadine hydrochloride, in particular to a pharmaceutical composition of amantadine hydrochloride and its neuroprotective medical application. Background technique [0002] Amantadine Hydrochloride (Amantadine Hydrochloride) is a symmetrical tricyclic amine, which can inhibit the penetration of the virus into the host cell, affect the uncoating of the virus, inhibit its reproduction, and play a role in the treatment and prevention of viral infection. [0003] Amantadine has a narrow antiviral spectrum and is mainly used for the prevention of Asian influenza A. It is ineffective against influenza B virus, rubella virus, measles virus, mumps virus and herpes simplex virus infection. Because it can pass through the blood-brain barrier after oral absorption, it will cause toxic and side effects in the central nervous system. [0004] So far, there has been no report on...

Claims

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Application Information

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IPC IPC(8): C07D311/58A61K31/352A61K31/13A61P25/00
CPCC07D311/58A61K31/13A61K31/352
Inventor 不公告发明人
Owner WENZHOU JUYICHUANG TECH CO LTD
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