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Pharmaceutical composition of aminoglutethimide and medicinal use of pharmaceutical composition of aminoglutethimide in neuroprotection

A technology for aminolutamide and neuroprotection, which is applied in the field of pharmaceutical compositions of aminolutamide, can solve problems such as no related reports on pharmaceutical compositions, and achieve outstanding substantive characteristics, significant progress, and improved neuroprotective effect. Effect

Inactive Publication Date: 2016-12-07
袁肇斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there has been no report on the correlation between the pharmaceutical composition of aminoglutethimide and neuroprotection

Method used

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  • Pharmaceutical composition of aminoglutethimide and medicinal use of pharmaceutical composition of aminoglutethimide in neuroprotection
  • Pharmaceutical composition of aminoglutethimide and medicinal use of pharmaceutical composition of aminoglutethimide in neuroprotection
  • Pharmaceutical composition of aminoglutethimide and medicinal use of pharmaceutical composition of aminoglutethimide in neuroprotection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0026] Sources of reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane were of analytical grade, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, of analytical grade, were purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.

[0027] Separation method: (a) crush the dried mint leaves (5kg), extract with 75% ethanol under heat reflux (20L × 3 times), combine the extracts, concentrate until no alcohol smell (4L), and then use petroleum ether (4L× 3 times), ethyl acetate (4L × 3 times) and water-saturated n-butanol (4L × 3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) step (a) Use D101 type macroporous resin to remove impurities from the n-butanol extract in (a), first elute with 8% ethanol for 6 column volumes, then use 70% ethanol for 8 column volumes, collect 70% eluate...

Embodiment 2

[0030] Example 2: Neuroprotection

[0031] 1. Materials and Instruments

[0032] The PC12 cell line was donated by the Experimental Center of Anhui University of Traditional Chinese Medicine. Aβ 1-40 Protein was purchased from Sigma Company. Compound (I) is self-made, and the HPLC normalized purity is greater than 98%. DMEM / F12 medium (imported packaging was purchased from Beijing Thermo Fisher Biochemical Products Co., Ltd. Newbom Calf Serum was purchased from Beijing Solarbio Science & Technology Co., Ltd. MTT was purchased from Amresco, USA. KGI AnnexinV-FITC cell apoptosis Death detection kit was purchased from Nanjing Kaiji Biotechnology Co., Ltd. Paraformaldehyde was purchased from China Pharmaceutical Group Chemical Reagent Co., Ltd. Bcl-2 polyclonal antibody was purchased from Wuhan Boster Biotechnology Co., Ltd. Bax polyclonal antibody was purchased from Beijing Boaosen Biotechnology Co., Ltd. Cyt-c polyclonal antibody was purchased from Wuhan Boster Biotechnology...

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Abstract

The invention discloses a pharmaceutical composition of aminoglutethimide and medicinal use of the pharmaceutical composition of the aminoglutethimide in neuroprotection. The pharmaceutical composition of the aminoglutethimide, provided by the invention, contains the aminoglutethimide and a natural product compound (1) with a novel structure represented by a formula I shown in the description separated from dried herba menthae leaves; the aminoglutethimide and the natural product compound (I) are relatively weak in neuroprotection action during independent acting; the neuroprotection action is remarkably improved when the aminoglutethimide and the natural product compound (I) are in joint action, so that the aminoglutethimide and the natural product compound (I) can be developed into drugs for neuroprotection; and compared with the prior art, the pharmaceutical composition of the aminoglutethimide has outstanding substantive features and marked progress.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of aminoglutethimide, in particular to a pharmaceutical composition of aminoglutethimide and its neuroprotective medical application. Background technique [0002] Aminoglutethimide is an adrenocortical hormone inhibitor and an antineoplastic drug. It has an inhibitory effect on the lyase system that converts cholesterol into pregnenolone, thereby blocking the synthesis of adrenal cortex hormones. It also has a certain inhibitory effect on other transformation processes of corticosteroid synthesis and metabolism. In peripheral tissues, it can inhibit the production of estrogen by blocking aromatase, thereby reducing the promotion effect of estrogen on breast cancer and inhibiting tumor growth. [0003] Aminoglutethimide can block androgen biosynthesis in two different parts of the adrenal cortex and extraglandular tissue, thereby acting as a drug adrenalectomy. In the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00A61K31/585A61K31/451A61P25/00
CPCC07J73/003A61K31/451A61K31/585A61K2300/00
Inventor 袁肇斌
Owner 袁肇斌
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