Compound used as anti-obesity agent
A compound and obesity technology, applied in the field of food chemistry, can solve the problems of α-glucosidase inhibition and other unknown inhibitory effects, and achieve excellent lipase inhibitory activity, inhibition of absorption, and high safety effects
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Embodiment 1
[0021] Embodiment 1: the preparation of formula I compound
[0022] Longjing green tea leaves were extracted with hot water at 90°C, and distilled water was added to the freeze-dried product to make it 10 mg / ml, followed by heating and dissolving. While heating to 60-70° C. in a water bath, 15 ml was added to 60 ml of Sephadex LH-20 (manufactured by GE HEALTHCARE BIOSCIENCES Co., Ltd.). Wash with 20% acetone (acetone: distilled water=2:8, v / v) 45ml, then with 60ml (1 column volume), then wash with 30% acetone (acetone: distilled water=3:7, v / v), 50% acetone (acetone: distilled water = 1:1, v / v) and 60% acetone (acetone: distilled water = 6:4, v / v) were eluted with 4 column volumes each, and each column volume was separated . Extract 2 ml from each 60 ml of 14 fractions in total, and supply 0.1 ml of 50% dimethyl sulfoxide aqueous solution (dimethyl sulfoxide: distilled water = 1:1, v / v) prepared by concentration under reduced pressure to the fat Enzyme inhibitory activity a...
Embodiment 2
[0024] Embodiment 2: biological activity test
[0025] The lipase activity was measured by using fluorescent 4-methylumbelliferone oleate (4-MUO; manufactured by Sigma) as a substrate and measuring the fluorescence of 4-methylumbelliferone produced by the reaction. implement. The buffer used in the determination contains 150mM NaCl, 1.36mM CaCl 2 13mM Tris-HCl (pH8.0). 4-MUO as a substrate was diluted 4,000-fold with the above-mentioned buffer in 0.1M DMSO solution, and then used for enzyme measurement. For lipase, 400 U of porcine pancreatic lipase (manufactured by Sigma) was prepared in the same manner as in the above-mentioned buffer. / ml solution for enzyme assay.
[0026] The enzyme reaction was started by adding and mixing 50 μl of 4-MUO buffer solution and 25 μl of distilled water (or sample aqueous solution) on a 96-well microplate at 25° C., and then adding 25 μl of lipase buffer solution. After reacting for 30 minutes, 100 µl of 0.1 M citrate buffer (pH 4.2) was ...
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