Polypeptide used for auxiliary diagnosis of malaria, detection member comprising polypeptide and detection kit

A technology of detection kits and devices, applied in the biological field, can solve the problems of inability to meet the high requirements of patients and examiners' skills and inspection requirements

Inactive Publication Date: 2016-06-08
302 MILITARY HOSPITAL OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the classic method for diagnosing malaria is still blood smear microscopy, which requires high skills and testing of examiners

Method used

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  • Polypeptide used for auxiliary diagnosis of malaria, detection member comprising polypeptide and detection kit
  • Polypeptide used for auxiliary diagnosis of malaria, detection member comprising polypeptide and detection kit
  • Polypeptide used for auxiliary diagnosis of malaria, detection member comprising polypeptide and detection kit

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Embodiment

[0066] Hereinafter, the present invention will be described more specifically through examples, but the technical scope of the present invention is not limited. Through the description of this specification, those skilled in the art can easily modify / change the present invention, and these are included in the technical scope of the present invention.

[0067] 1.30 Preparation and Confirmation of Peptides

[0068] The 30 peptide used in the examples has the amino acid sequence shown in SEQ ID NO: 1 and was synthesized by Shanghai Jier Biochemical Co., Ltd. The characterization of the polypeptide can confirm that the polypeptide was synthesized by hydrogen spectrum and mass spectrum. Liquid chromatography detection purity: 96.67% (area normalization method): mass spectrometry detection (ESI-MS): calculated molecular weight: 3501.81, test value 3502.12.

[0069] 2. Fabrication of Detection Devices

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Abstract

The present invention relates to a polypeptide used for auxiliary diagnosis of malaria, a detection member comprising the polypeptide and a detection kit. The polypeptide comprises an amino acid sequence shown in SEQIDNO: 1. The polypeptide, the detection member containing the polypeptide, and the detection kit can be used in the auxiliary diagnosis of malaria.

Description

technical field [0001] The invention mainly relates to a polypeptide used for auxiliary diagnosis of malaria, a detection device and a detection kit containing the polypeptide, and belongs to the field of biotechnology. Background technique [0002] Malaria is an acute infectious disease and the most serious mosquito-borne parasitic disease to humans in the world. The pathogen is Plasmodium, and the symptoms are periodic chills and fever. Due to the complex life cycle of Plasmodium and stage-specific antigens, the prevention and control of malaria has become one of the worldwide problems. Among them, falciparum malaria infects 300-500 million people every year and kills millions of people. In November 2011, according to the latest World Health Organization statistics, there were 216 million malaria cases and 655,000 deaths. Plasmodium is mainly distributed in the tropical and subtropical countries of the earth, namely Africa, Southeast Asia, South America and other regions...

Claims

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Application Information

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IPC IPC(8): C07K14/445C07K16/20C12N15/30C12N15/63G01N33/68
CPCY02A50/30
Inventor 李伯安侯俊张健王晗毛远丽
Owner 302 MILITARY HOSPITAL OF CHINA
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