A real-time detection device for cell metabolites based on a microfluidic chip

Abstract

The present invention provides a real-time cell metabolite detection device based on a microfluidic chip. The device comprises the microfluidic chip, an automatic sampling assembly, a pretreatment and enrichment assembly and a detector. A channel configured with a cell culture chamber is disposed inside the microfluidic chip, and the automatic sampling assembly capable of collecting a sample from an outlet of the channel is disposed at a periphery of the microfluidic chip. Downstream of the automatic sampling assembly is connected the pretreatment and enrichment assembly capable of desalting a cell metabolite collected and enriching a target product. The detector is disposed at an outlet of the pretreatment and enrichment assembly, wherein the detector carries out in-line detection of the target product. The detection device of the present invention integrates the microfluidic chip, automatic sampling assembly, pretreatment and enrichment assembly and detector to allow real-time and in-line detection and analysis of a biochemical reaction as well as a drug metabolic process on the microfluidic chip.

Description

technical field [0001] The invention relates to the technical field of cell research, in particular to a real-time detection device for cell metabolites based on a microfluidic chip. Background technique [0002] As we all know, compared with traditional cell research platforms, microfluidic chip technology has attracted widespread attention due to its low reagent consumption, easy integration, high throughput, easy operation and good reproducibility. In recent years, animal experiments have gradually been opposed by humans, and microfluidic chip technology is one of the most potential methods to replace animal experiments, and has broad application prospects. The channels in microfluidic chips are generally micron-scale channels comparable to the size of cells, which allows researchers not only to precisely manipulate cells, but also to reduce the amount of cells used and the consumption of reagents. In the prior art, using the characteristics of the microfluidic chip, the...

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Problems solved by technology

In addition, the detection and analysis devices in the prior art still have many deficiencies in product enrichment, detection limit and anti-interference, which make the complex biochemical reactions and drug metabolism on the microfluidic chip are greatly restricted.

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