Two-dimensional diffusion-ordered nuclear magnetic resonance spectroscopy method used for any magnetic field environments

Abstract

The invention discloses a two-dimensional diffusion-ordered nuclear magnetic resonance spectroscopy method used for any magnetic field environments and relates to nuclear magnetic resonance spectroscopy detection methods. The method includes the steps of 1), measuring pi / 2 nonselective radiofrequency pulse width and (pi / 2) solvent selective radiofrequency pulse width required by sample stimulation; 2), importing a nuclear magnetic resonance pulse sequence on a nuclear magnetic resonance spectrometer; 3), turning on an intermolecular zero-quantum coherence signal selection module, a time-invariant evolution module and a diffusion order module of the nuclear magnetic resonance pulse sequence and setting experiment parameters of all modules; 4), executing data sampling; 5), performing data post-processing to obtain high-resolution two-dimensional diffusion-ordered spectroscopy. The two-dimensional diffusion-ordered nuclear magnetic resonance spectroscopy method used for any magnetic field environments does not need any shimming operation, a sample preprocessing process and any special hardware devices, is simple, convenient and feasible and is applicable to any conventional nuclear magnetic resonance spectrometers.

Description

technical field [0001] The invention relates to a nuclear magnetic resonance (NMR, Nuclear Magnetic Resonance) spectroscopy detection method, in particular to a NMR two-dimensional diffusion sorting spectrum method for any magnetic field environment, which relates to the separation and composition analysis of mixture components in any magnetic field environment. Background technique [0002] Nuclear magnetic resonance (Nuclear Magnetic Resonance, NMR), as an important detection and analysis method, has a wide range of applications in the fields of physics, chemistry, biology, and materials due to its accuracy, high efficiency, non-destructive testing and many other advantages. For the detection of NMR of complex mixtures, the spectral peaks of various substances are all presented on the same spectrum, which often leads to crowded spectral peaks and it is difficult to obtain the correct composition assignment. For this reason, it is generally required to separate and purify t...

AI Technical Summary

Problems solved by technology

However, in many cases, the uniformity of the magnetic field cannot be absolutely guaranteed. Even the shimming technology cannot completely eliminate the magnetic field inhomogeneity caused by various samples and environmental factors during the experiment.

For example, in the research of some biomedical mixture samples, the sample molecules often have specific activity and function only under the condition of tissue coexistence, and the internal magnetic susceptibility of this tissue will cause the inhomogeneity of the magnetic field, so the existing diffusion sorting spectrum methods are often difficult to apply to such mixture systems

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