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Method for efficiently and quickly extracting paclitaxel, cephalomannine and 10-DAB III from Taxus chinensis

A technology of cephalomannine and paclitaxel, which is applied in the direction of organic chemistry, can solve the problems of reducing the number of resin column cycles, long extraction time, and increasing the steps of organic weak acid aqueous solution, and achieves convenient operation, simple production equipment, and production conditions easy effect

Inactive Publication Date: 2016-06-15
CHONGQING BEISHENG PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: 1. The extraction time is long, 4 to 48 hours; 2. The filtrate filtered by the filter plate at the lower part of the extraction tank contains many fine particles, which will block the resin column when entering the resin column, reducing the recycling of the resin column. times; ③ using organic weak acid aqueous solution to extract and increase the step of subsequent treatment of organic weak acid aqueous solution

Method used

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  • Method for efficiently and quickly extracting paclitaxel, cephalomannine and 10-DAB III from Taxus chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Extraction of 10-DABⅢ:

[0035] The branches and leaves of Taxus chinensis are dried in the shade and then crushed to 0.1mm to obtain yew powder; : 5 ratio, add 10-DABⅢ extractant in the yew powder, then carry out ultrasonic extraction under the action of ultrasonic waves with an ultrasonic frequency of 20KHz, and the extract solution enters a rotating speed of 6000r / min after filtering through the filter plate under the ultrasonic extraction tank. Centrifuge in a tubular centrifuge to obtain the centrifugal supernatant and sediment. The sediment is treated as waste residue. The centrifugal supernatant enters the macroporous resin column equipped with AB-8 for adsorption, and the solution after adsorption continues to enter the ultrasonic extraction tank. extract and filter to obtain filtrate and filter residue, and the filtrate is used as the extractant of the next batch of materials, and the filter residue is placed in an ultrasonic extraction tank; the adsorbed res...

Embodiment 2

[0041] (1) Extraction of 10-DABⅢ:

[0042] The branches and leaves of Taxus chinensis are dried in the shade and then crushed to 0.1mm to obtain yew powder; : the ratio of 100, add 10-DABⅢ extractant to the yew powder, and then carry out ultrasonic extraction under the action of ultrasonic waves with an ultrasonic frequency of 50KHz. Centrifuge in a tubular centrifuge to obtain the centrifugal supernatant and sediment. The sediment is treated as waste residue. The centrifugal supernatant enters the macroporous resin column equipped with D101 for adsorption, and the solution after adsorption continues into the ultrasonic extraction tank. The entire ultrasonic extraction time is 6 hours, and then the material in the ultrasonic extraction tank is filtered to obtain the filtrate and filter residue, the filtrate is used as the extractant for the next batch of materials, and the filter residue is placed in the ultrasonic extraction tank; the resin column after adsorption is to be ad...

Embodiment 3

[0048] (1) Extraction of 10-DABⅢ:

[0049] The branches and leaves of Taxus chinensis are dried in the shade and then crushed to 0.5mm to obtain the yew powder; : the ratio of 50, 10-DABⅢ extractant in the yew powder, then carry out ultrasonic extraction under the ultrasonic wave effect of 35KHz in ultrasonic frequency, the total time of ultrasonic treatment is 4 hours, and extracting liquid is carried out through the filter plate under the ultrasonic extraction tank After filtration, it enters a tubular centrifuge with a rotating speed of 10000r / min for centrifugation to obtain the centrifugal supernatant and sediment. The sediment is treated as waste residue, and the supernatant enters the macroporous resin column equipped with HPD300 for adsorption. The solution enters the ultrasonic extraction tank to continue the extraction, and the material in the ultrasonic extraction tank is filtered to obtain the filtrate and filter residue. The filtrate is used as the extractant for ...

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Abstract

The invention discloses a method for efficiently and rapidly extracting paclitaxel, cephalomannine and 10-DABⅢ from yew, which comprises dissolving yew powder in 10-DABⅢ extractant, performing ultrasonic extraction for the first time, and then collecting by centrifugation Centrifugal supernatant Ⅰ, the centrifugal supernatant Ⅰ is eluted after being saturated and adsorbed by the macroporous resin, and the eluate Ⅰ is collected and concentrated to obtain 10-DABⅢ extract. Collect the filter residue and filtrate after ultrasonic extraction; dissolve the filter residue collected in step a in paclitaxel extractant, collect the supernatant II after ultrasonic extraction and centrifugation, and collect the supernatant II after saturated adsorption by macroporous resin The eluate II is concentrated to obtain the extract containing paclitaxel and cephalomannine; it has the characteristics of simple preparation process, production equipment and operation steps, short extraction time, recyclable materials, and long use time.

Description

technical field [0001] The invention relates to the technical field of separating and extracting active ingredients from plants, in particular to a method for efficiently and rapidly extracting paclitaxel, cephalomannine and 10-DABⅢ from yew. Background technique [0002] Taxane compounds are a class of compounds extracted from the bark, roots and leaves of the higher gymnosperm Taxus chinensis, mainly containing paclitaxel, cephalomannine, 10-DABⅢ, 7-xylosyl paclitaxel, etc. Many have been proven to have cytotoxicity and anti-tumor activity, but the natural yew resources used to extract paclitaxel are limited, so the production and supply of natural paclitaxel raw materials are limited, which is far from meeting the clinical application of paclitaxel. Now people plant yew trees on a large scale to extract taxanes such as paclitaxel, cephalomannine, 10-DABⅢ, 7-xylosyl paclitaxel, how to make full use of yew resources and get more effective Matter matters. Existing methods ...

Claims

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Application Information

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IPC IPC(8): C07D305/14
CPCC07D305/14
Inventor 潘先文潘敬坤夏涛张龙
Owner CHONGQING BEISHENG PHARMA TECH CO LTD
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