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A rice anther-specific expression promoter osanth3 and its application

An anther-specific, promoter technology is applied in the fields of biotechnology and plant genetic engineering to achieve the effects of improving fertility, blocking gene drift, and reducing ecological risks

Active Publication Date: 2020-03-27
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the genetic drift of transgenic crops and its possible environmental consequences are potential risks to the ecological safety of genetically modified crops

Method used

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  • A rice anther-specific expression promoter osanth3 and its application
  • A rice anther-specific expression promoter osanth3 and its application
  • A rice anther-specific expression promoter osanth3 and its application

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0036] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0037] The experimental methods in the following examples are conventional methods unless otherwise specified. The biochemical reagents, carrier consumables, etc. used in the following examples are all commercially available products unless otherwise specified.

[0038] Acquisition of OsAnth3 Promoter Containing Restriction Sites

[0039] Step 1. Design of primers

[0040] According to the whole genome sequence of rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of the rice OsAnth3 gene, and the enzyme cutting sites of the primers were designed according to the characteristics of the selected vector and target gene.

[0041...

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Abstract

The invention provides a rice anther specific expression promoter OsAnth3 and application thereof. The invention also provides an expression cassette containing the promoter, a recombinant expression vector and a method for obtaining corresponding host bacteria and transformants by using the same. As verified by staining experiments, the rice anther-specific expression promoter OsAnth3 of the present invention can drive the strong expression of the target gene in rice anthers, while there is almost no expression in other parts, which proves that the promoter of the present invention has strong tissue specificity. Applying it to the breeding of transgenic varieties can change the growth characteristics of anther parts with minimal metabolic burden, and has significant potential social and economic value. The present invention applies it to plant genetic engineering, drives the specific expression of target genes in crop anthers, uses the promoter of the present invention in conjunction with genes that have the function of inhibiting or interfering with anther growth, interferes with the growth and development of crop anthers, and is used for hybridization Breeding research; blocking gene drift and preventing and controlling ecological risks that may be caused by genetically modified crops.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a rice anther-specific expression promoter OsAnth3 and its application. The promoter can drive the specific expression of a target gene in rice anthers in rice transgenic engineering. Background technique [0002] Rice is one of the most important food crops in the world, and it is also a good model organism for monocotyledonous plants. In today's growing population and decreasing arable land, increasing grain yield per unit area has become one of the important ways to ensure food security in our country. Improving rice production has always played an important role in ensuring my country's food security. The utilization of heterosis is an important way to increase rice yield and improve crop varieties. However, the conventional breeding methods of hybrid rice have limited germplasm resources, long transfer cyc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/84C12N1/21A01H5/00A01H6/46C12R1/01
CPCC12N15/113C12N15/8205C12N15/8231C12N2310/10C12N2800/60
Inventor 李娟杨剑波魏鹏程李浩李莉杨亚春许蓉芳秦瑞英
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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