A rapid quantitative detection method for cocaine suitable for on-site detection
A quantitative detection method and on-site detection technology, applied to measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of lack of rapid quantitative detection methods for cocaine, and achieve the effects of improving fluorescence quenching efficiency, simple operation, and avoiding interference
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Embodiment 1
[0049] A cocaine rapid quantitative detection method suitable for on-site detection, comprising the following steps:
[0050] (1) Prepare a paper-based detection device as described above, wherein the paper is made of cellulose filter paper, and the inner diameter of the circular hydrophilic detection area is 3 mm, and 6 μL of a mixture of PEI-UCPs and ACA-1 is added to each hydrophilic detection area. Solution, wherein the concentration of the water-soluble up-conversion fluorescent nanomaterial PEI-UCPs is 0.5 mg / mL and the concentration of single-stranded nucleic acid ACA-1 is 2 μM;
[0051] (2) Prepare AuNPs-ACA-2 solution as described above;
[0052] (3) Concentration optimization of AuNPs-ACA-2 solution: 3 μL of 10 μM cocaine solution and 3 μL of AuNPs-ACA-2 solution of different concentrations were added to the hydrophilic detection area of the paper-based detection device described in step (1) ( Concentrations were 0, 0.07, 0.105, 0.14, 0.175, 0.21, 0.28, 0.315μM), ...
Embodiment 2
[0063] A cocaine rapid quantitative detection method suitable for on-site detection, comprising the following steps:
[0064] (1) Prepare a paper-based detection device as described above, wherein the paper is made of cellulose filter paper, and the inner diameter of the circular hydrophilic detection area is 2 mm, and 2.6 μL of PEI-UCPs and ACA-1 are added to each hydrophilic detection area. The mixed solution, wherein the concentration of the water-soluble up-conversion fluorescent nanomaterial is 0.5 mg / mL and the concentration of single-stranded nucleic acid ACA-1 is 0.5 μM;
[0065] (2) Prepare AuNPs-ACA-2 solution as described above;
[0066] (3) Concentration optimization of AuNPs-ACA-2 solution: add 1.3 μL of 10 μM cocaine solution and 1.3 μL of different concentrations of AuNPs-ACA-2 to the hydrophilic detection area of the paper-based detection device described in step (1). solution (0, 0.07, 0.105, 0.14, 0.175, 0.21, 0.28, 0.315μM respectively), reacted at 20°C f...
Embodiment 3
[0072] A cocaine rapid quantitative detection method suitable for on-site detection, comprising the following steps:
[0073] (1) Prepare a paper-based detection device as described above, wherein the paper is made of cellulose filter paper, and the inner diameter of the circular hydrophilic detection area is 6 mm, and 24 μL of a mixture of PEI-UCPs and ACA-1 is added to each hydrophilic detection area Solution, wherein the concentration of water-soluble up-conversion fluorescent nanomaterial is 0.5 mg / mL and the concentration of single-stranded nucleic acid ACA-1 is 4 μM;
[0074] (2) Prepare AuNPs-ACA-2 solution as described above;
[0075] (3) Concentration optimization of AuNPs-ACA-2 solution: 12 μL of 10 μM cocaine solution and 12 μL of AuNPs-ACA-2 solution of different concentrations were added to the hydrophilic detection area of the paper-based detection device described in step (1) ( Concentrations were 0, 0.07, 0.105, 0.14, 0.175, 0.21, 0.28, 0.315μM), reacted at ...
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