GABAergic neuron conditional knockout gene PGC-1 alpha mouse model and construction method thereof

A PGC-1 and mouse model technology, applied in biochemical equipment and methods, microbe measurement/inspection, animal husbandry, etc., can solve the problems of decreased survival rate and obstruction, and achieve the effect of avoiding the decline of survival rate
CN105684993AInactive Publication Date: 2016-06-22JIANGSU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2016-06-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a GABAergic neuron conditional knockout gene PGC-1 alpha mouse model and a construction method thereof and belongs to the field of animal models and application thereof. The construction method includes the steps that a PGC-1 alpha <flox / flox> mouse is obtained in the first place; then, a PGC-1 alpha <flox / +> is mated with a C57BL / 6J, and the PGC-1 alpha <flox / +> is obtained, then the PGC-1 alpha <flox / +> and the C57BL / 6J are mated with a Dlx5 / 6<Cre-IRES-EGFP> mouse, and the full knockout D1x5 / 6< Cre-IRES-EGFP >, PGC-1 alpha <flox / flox>, semi knockout D1x5 / 6< Cre-IRES-EGFP > and a PGC-1 alpha <flox> mouse are obtained; as a result, a gene knockout mouse on GABAergic internuncial neuron conditional knockout PGC-1 alpha is prepared, and a credible animal model is provided for mechanism research on neurodevelopment and neurodegenerative diseases.
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Description

technical field

[0001] The invention relates to a GABAergic neuron conditional knockout gene PGC-1α mouse model and a construction method thereof, belonging to the field of animal models and applications thereof. Background technique

[0002] Peroxisome proliferator-activated receptor γ coactivator-1α (peroxisome proliferator-activated receptor-γcoactivator-1α, PGC-1α) as a transcriptional coactivator regulates the synthesis of mitochondria and the expression of genes related to oxygen metabolism in mitochondria plays a key role. The transcriptional co-activator PGC-1α recruits other specific transcription factors, induces histone deacetylation, affects chromosome structure changes and initiates transcription (LinJ, WH., TarrPT, ZhangCY, et al. Transcriptional co-activator PGC-1alpha drives the formation of slow-twitch muscle fibres. Nature, 2002, 418:797-801.).

[0003] In peripheral tissues, PGC-1α is defined as a metabolic regulatory target for its ability to induce mit...

Claims

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