Serum markers for preoperative detection of microvascular invasion in liver cancer
A microvessel and serum technology, applied in the field of serum markers, can solve the problems of insufficient specificity, ineffective recognition of proteins, and large differences
Active Publication Date: 2021-01-19
李江涛
View PDF1 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
In recent years, more and more scholars have studied MVI-related markers from the level of molecular biology, but the specificity and accuracy of these markers are not ideal, and they are far from clinical diagnosis
In the past, the screening method for liver cancer antigens was mainly based on two-dimensional electrophoresis-mass spectrometry analysis technology, but this technology has some shortcomings: 1. The specificity of identifying antigens by two-dimensional electrophoresis between normal liver tissue and liver cancer tissue and mass spectrometry analysis is insufficient;2. . There are large differences between individuals, and liver cancer has multiple stages, so the result deviation is large; 3. Some important proteins cannot be effectively identified, and the sensitivity is poor
At present, there is still a lack of an efficient and accurate method for the preoperative detection of liver cancer MVI.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0012] The screening and reverse verification process of the serum markers of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0013] The present invention collects and sorts out 160 liver cancer tissue sample banks, including preoperative peripheral blood, postoperative liver cancer tissue, adjacent normal tissue, and related clinical case data. refer to figure 1 The flow shown is for reverse serum-proteomics experimental research.
[0014] 1. Samples to be collected, each case contains:
[0015] 1) Tumor tissue paraffin block × 1;
[0016] 2) Fresh specimens of tumor tissue x 1 are stored in a -80°C refrigerator;
[0017] 3) Paraffin block of normal liver tissue adjacent to the tumor × 1;
[0018] 4) A fresh specimen of normal liver tissue adjacent to the tumor x 1 is stored in a -80°C refrigerator;
[0019] 5) Store the plasma supernatant × 1 in a -80°C refrigerator after two centrifugations;
[0020] 6) After ce...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The present invention discloses serum markers for preoperative detection of microvascular invasion of liver cancer. For the first time, reverse serum-proteomics technology is used to study related antigens and antibodies of liver cancer MVI in vivo, and serum markers for preoperative detection of liver cancer microvascular invasion are determined. It provides an effective detection method for the early diagnosis of MVI before clinical operation.
Description
technical field [0001] The invention relates to a serum marker, in particular to a serum marker used for preoperative detection of liver cancer microvessel invasion. Background technique [0002] Microvascular invasion (MVI) in hepatocellular carcinoma refers to the observation under the microscope that nests of cancer cells can be seen in the vascular lumen lined with endothelial cells. Occult metastases produced by liver cancer cells invading microvessels are one of the important reasons for postoperative recurrence of liver cancer. Literature has confirmed that MVI is an independent indicator for predicting recurrence and evaluating prognosis after liver cancer resection. American Joint Committee on Cancer (AJCC) guidelines have taken MVI as one of the important factors for TMN staging of liver cancer. Currently, the diagnosis of MVI is based on pathological examination of postoperative or biopsy needle specimens. At the imaging level, some scholars used Gadoxetata Diso...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/573
CPCG01N33/573G01N33/57438G01N33/57484
Inventor 李江涛金赟于源泉周佳乐钱程明杨俊杰张小小
Owner 李江涛