Identification method of phytophthora parasitica var.nicotianae physiological race

Abstract

The invention discloses an identification method of a phytophthora parasitica var.nicotianae physiological race, and belongs to the technical field of plant pathogen identification. According to the identification method disclosed by the invention, the phytophthora parasitica var.nicotianae physiological race is identified by virtue of a tissue-culture vessel method, so that cultivating and impregnating environmental conditions are more stable; the identification method is beneficial for promoting the growth of tobacco and pathogenic bacteria and for greatly shortening a seedling cycle and an impregnating cycle; meanwhile, the identification method can also avoid interference of infectious microbes and other physiological races on an identification result; the identification method is high in throughput and is capable of saving manpower and material resources to the greatest extent, so that the high-throughput, rapid and accurate identification of the phytophthora parasitica var.nicotianae physiological race is achieved. Compared with a field method and a greenhouse method, the identification method is rapid to identify, more accurate, stable and reliable in result and is relatively high in application value. Meanwhile, a new thinking is provided for the physiological race identification of other diseases and pathogens of the tobacco.

Description

technical field [0001] The invention relates to a method for identifying physiological races of tobacco blackleg pathogen, belonging to the technical field of plant pathogen identification. Background technique [0002] Tobacco black shank is a devastating soil-borne oomycete disease worldwide. In the late 1980s, with the continuous expansion of tobacco planting area in my country, tobacco black shank disease spread rapidly. Due to the differences in the pathogenicity of different races of Tobacco black shank, close monitoring of the races of Black shank in tobacco fields is required. So far, there are 5 physiological races of Tobacco black shank pathogens reported in the world, of which 4 are reported in the United States, namely race 0, 1, 3 and 4, and 1 species is reported in Africa, namely race 2 No. 1 race, but only No. 0 and No. 1 races are found in my country. [0003] The invention patent with publication number CN101671720A discloses a method for identifying physi...

AI Technical Summary

Problems solved by technology

Although this method can preliminarily identify the physiological race of Tobacco black shank pathogen, the accuracy of the test results still needs to be verified because the field cultivation is greatly affected by environmental factors.

Generally, the identification of physiological races of tobacco black shank is carried out by root inoculation under greenhouse conditions, but this method needs to be equipped with greenhouse facilities, and the strain inoculation requires at least 7 to 8 week-old tobacco seedlings (seedlings need to be transplanted before inoculation) , time-consuming operation

Images