Preparing method for high-purity grifola frondosa polyphenol component

Abstract

The invention relates to a preparing method for a high-purity grifola frondosa polyphenol component, and belongs to the field of functional factors of functional food and medicine.The preparing method includes the steps that a grifola frondosa polyphenol crude component is prepared through an ultrasonic-assisted extraction technology, biomacromolecules such as polysaccharide and protein are removed with an ultrafiltration membrane retention technology, and finally various high-purity grifola frondosa polyphenol compound monomers are prepared by adopting the macroporous adsorbent resin NKA-II type column chromatography-preparative high performance liquid chromatography series separation and purification technology.The preparing method is fast and efficient, and seventeen grifola frondosa polyphenol compound monomers can be prepared at the same time and high in purity.The prepared grifola frondosa polyphenol compound monomers has a high DPPH free radical and hydroxyl free radical scavenging rate and high reducing power, activity of alpha-glucosidase can be restrained by most grifola frondosa polyphenol compound monomers, and the grifola frondosa polyphenol compound monomers have the physiological effects of effectively protecting human body cell tissue and heart and cerebral vessel circulating system, resisting cancer, delaying ageing and assisting in reducing blood sugar.

Description

technical field [0001] The invention relates to a preparation method of high-purity grifola frondosa polyphenol components, belonging to the field of functional factors and medicines of functional foods. Background technique [0002] Grifolafrondosa (Grifolafrondosa) belongs to the fungus of the genus Treeflower of the Basidiomycotina Phylomycetes, Polyporaceae, Polyporaceae, and mainly grows in subtropical to temperate regions. In my country, it is mainly distributed in Fujian, Hebei, Jilin, Guangxi and Sichuan. . As a precious edible and medicinal fungus, the multiple nutrients and physiological functional components of Grifola frondosa rank first among all kinds of edible fungi, and it is known as the "Prince of Edible Fungi" and "North China Ginseng". Relevant studies have shown that Grifola frondosa has many health care effects such as enhancing immunity, inhibiting tumors, anti-virus, anti-oxidation and anti-aging, stabilizing blood pressure, lowering blood sugar and b...

AI Technical Summary

Problems solved by technology

Chen Xiangdong et al. used weakly polar macroporous adsorption resin to separate and prepare polyphenolic components from the crude extract of Grifola frondosa mycelium. The obtained components were brown powder products, which were verified to have antioxidant, antibacterial and α -Amylase inhibition and other activities, but the polyphenols of Grifola frondosa produced are a mixture, and the components of the polyphenols of Grifola frondosa have not been further purified and identified, and the polyphenol components of Grifola frondosa need to be further separated and purified ( Chen Xiangdong, Liu Xiaowen, Wu Wutong. Extraction and activity of polyphenols from Grifola frondosa. Journal of Food and Biotechnology, 2005,24(4):26-30.)

Images