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Molecular markers and their application of the main QTL of heading stage in rice

A molecular marker and heading stage technology, which is applied in the field of molecular genetics and breeding, can solve the problems of limiting the application of rice heading stage and the number of heading stages, etc., and achieves the effect of saving time and reducing labor costs.

Active Publication Date: 2018-03-27
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of cloned heading date is too small, which greatly limits the application of rice heading date in production practice. Therefore, it is urgent to strengthen the isolation of rice heading date quantitative trait loci

Method used

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  • Molecular markers and their application of the main QTL of heading stage in rice
  • Molecular markers and their application of the main QTL of heading stage in rice
  • Molecular markers and their application of the main QTL of heading stage in rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Obtaining of Molecular Markers Closely Linked to Heading Stage Main Effect Genes

[0021] (1) Positioning groups and test methods

[0022] 1. Positioning groups

[0023] Zhan Xiaodeng and others initially positioned qHD5 between molecular markers RM17788 and RM5374. On this basis, the present invention further expands the positioning group, adopting the BC 4 f 2 BC 4 f 3 High generation segregation groups.

[0024] The present invention utilizes BC 4 f 3 The segregating populations were first genetically analyzed for qHD5, as figure 2 As shown, the segregation ratio of early heading and late heading is consistent with 1:3 (χ 2 =1.0159, P=0.3135>0.05), indicating that the gene is controlled by a single Mendelian factor, and late heading is dominant over early heading. figure 1 Shown is a comparison of rice phenotypes containing DTH5 and dth5.

[0025] The present invention utilizes BC 4 f 3 More than 2000 extreme recessive individuals in the high-...

Embodiment 2

[0036] Embodiment 2 (Utilize closely linked molecular marker H70 and 301K to identify, select the method for the suitable heading date variety of rice)

[0037] Specific steps are as follows:

[0038] (1) Extraction of DNA: Get the young leaves of rice, and extract genomic DNA by CTAB method;

[0039] (2) Establishment of a standard PCR amplification system: use any one or two pairs of labeled primers in H70 and 301K, and use the whole genome DNA of a single rice plant to be tested as a template to perform PCR amplification;

[0040] (3) Detection and analysis of amplified DNA fragments: PCR products are detected by 8% polyacrylamide gel electrophoresis. If the marker H70 can amplify a 171bp fragment, it indicates that the single plant genotype is DTH5, and its phenotype is late heading If a 165bp fragment can be amplified, it indicates that the individual plant genotype is dth5, and its phenotype is early heading; if a heterozygous band type can be amplified, it indicates th...

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Abstract

The invention discloses a molecular marker of the main effect QTL of rice heading stage and an application thereof. The molecular markers are H70 and 301K, wherein the H70 primer pair consists of the sequences described in SEQ ID No.1 and SEQ ID No.2; the 301K primer pair consists of the sequences shown in SEQ ID No.3 and SEQ ID No.4 ; These two molecular markers are closely linked with the heading date QTL, which can be used for identification and breeding of rice varieties for heading date. The invention also discloses a method for identifying and breeding rice heading date by using the molecular marker. In the present invention, for the first time, the main QTL for controlling heading date located on the short arm of chromosome 5 has been fine-mapped, and closely linked InDel markers H70 and 301K have been obtained. Only by detecting the characteristics of the amplified bands of these markers, the presence or absence of the variation of the main QTL for the identification of the heading stage can be judged to predict the heading stage phenotype of rice and guide the breeding work for improving the heading stage of rice.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, in particular, it relates to a molecular marker of a major QTL for heading stage in rice and an application thereof. Background technique [0002] Rice is one of the most important food crops in the world. Rice is grown in more than 110 countries and regions around the world. Nearly half of the population in my country eats rice as a staple food. Rice production plays an important role in ensuring food security in my country. However, with the development of modern agricultural production and society, intensive and modern cultivation techniques have put forward higher requirements for the cultivation characteristics of rice, especially regional adaptability. Traditional rice varieties can no longer meet the production needs. High-quality, wide-adapted new varieties have become the common goal of rice breeders. Heading date is one of the important agronomic traits of rice, and the length...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 占小登孙滨程式华曹立勇张迎信沈希宏
Owner CHINA NAT RICE RES INST