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Suppression method of infectious disease of crustaceans

A technology for crustaceans and infectious diseases, applied in the direction of antiviral agents, anti-infective drugs, single-celled algae, etc., can solve problems such as environmental adverse effects, antibiotic residues, and production of resistant bacteria, so as to improve immunity, suppress infectious diseases, The effect of stable supply

Active Publication Date: 2020-03-03
KANEKA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, since crustaceans are raised at a high density, once an infectious disease occurs, all or nearly all of them will die.
Therefore, antibiotics are sometimes used in the cultivation of crustaceans. However, in addition to the problem of antibiotics remaining in crustaceans, there are also problems such as adverse effects on the environment and generation of resistant bacteria.
Moreover, common antibiotics have the disadvantage that they are mainly effective against bacteria rather than resistant bacteria, and are ineffective against viruses and fungi. For viruses and fungi, special antibiotics are required

Method used

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  • Suppression method of infectious disease of crustaceans
  • Suppression method of infectious disease of crustaceans
  • Suppression method of infectious disease of crustaceans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1: the cultivation experiment of Penaeus japonicus

[0086] (1) Preparation of feed

[0087] Parachlorella kessleri KNK-A001 strain (Accession No.: FERM BP-22256) was inoculated into the sterilized liquid medium, shaded with aluminum foil, and pre-cultivated at 30° C. for 72 hours. Next, add the pre-culture medium to a larger volume of sterilized liquid medium, shield it with aluminum foil, and then cultivate it for 143 hours under the conditions of an internal temperature of 30°C, an aeration rate of 2L / min, a stirring speed of 450rpm, and a pH of 6-7.

[0088] Next, the culture solution was dried with a double-drum dryer, and the resulting dried aggregate was pulverized with a feather mill to obtain a dried cell product of the KNK-A001 strain.

[0089] In addition, Parachlorella beyerinckii species algae (product name "Beyerinckii", available from Mitsui & Co., Ltd.) were used in the same experiment as algae belonging to the genus Pseudochlorella.

[0090...

Embodiment 2

[0095] Embodiment 2: the cultivation experiment of Penaeus japonicus

[0096] Feed containing 0.02% by mass (200 ppm) or 0.05% by mass (500 ppm) of Parachlorella kessleri KNK-A001 strain (Accession No.: FERM BP-22256) was prepared in the same manner as in Example 1 (1). In addition, for comparison, only the above-mentioned feed for shrimp was used as a control.

[0097] Moreover, the cultivation experiment was performed similarly to Example 1 (2). show the result in figure 2 . figure 2 Among them, "*" indicates a significant difference at p<0.05 when the survival rate of Penaeus japonicus on the 14th day since infection with the virus was performed, and "**" indicates a significant difference at p<0.01.

[0098] according to figure 2 The results can prove that in the group fed with the feed compounded with algae of the genus Pseudochlorella, compared with the group fed with common feed, the survival rate was significantly improved, and viral infectious diseases could be...

Embodiment 3

[0099] Embodiment 3: the cultivation experiment of Penaeus japonicus

[0100] In Example 1, the same experiment was carried out using smaller Penaeus japonicus. That is, in the same manner as in Example 1 (1), 0.01% by mass (100ppm), 0.02% by mass (200ppm) or 0.05% by mass (500ppm) of feed. In addition, for comparison, only the above-mentioned feed for shrimp was used as a control. And in Example 1 (2), except having used the japonicus of body weight about 1.5g, and the number of the japonicus in each water tank was 25 tails, the cultivation experiment was performed similarly. show the result in image 3 .

[0101] Such as image 3 As shown in the results, even when the amount of inoculated virus liquid relative to the body weight was larger, the survival rate of the three groups fed with the feed containing algae of the genus Pseudochlorella was significantly higher than that fed only with the commercial feed of the control group. It should be noted that, in the same m...

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Abstract

An object of the present invention is to provide a method for suppressing infectious diseases that have no effective countermeasures among crustacean infectious diseases and that will cause all deaths once they occur in a breeding farm. The method of suppressing infectious diseases caused by crustaceans of the present invention is characterized by including a step of causing the crustaceans to ingest 0.001 g / kg body weight or more of an algae body of the genus Parachlorella per day.

Description

technical field [0001] The present invention relates to a method for suppressing infectious diseases of crustaceans by effectively improving the immunity of crustaceans themselves. Background technique [0002] For edible crustaceans, although there are naturally caught ones, most of them are farmed in order to achieve stable supply and high efficiency. Moreover, recently, "aquaculture fishery" in which crustaceans are raised to the larval stage and fully grown crustaceans are caught after releasing seedlings has become very popular. [0003] However, in the farming of crustaceans, since they are raised in a high-density state, once an infectious disease occurs, all or nearly all of them will die. Therefore, antibiotics are sometimes used for farming of crustaceans. However, in addition to the problem of antibiotics remaining in crustaceans, there are also problems of adverse effects on the environment and generation of resistant bacteria. Moreover, common antibiotics have...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/05A23K10/30A23K50/80A61P31/00A61P31/12C12N1/12C12N15/09
CPCA23K10/16A23K10/18A23K50/80A61K36/05A61P31/00A61P31/12C12N1/12
Inventor 山下宪司小田达也
Owner KANEKA CORP
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