Method for breaking secondary dormancy of morus alba seeds
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A technology for seeds and mulberry trees is applied in the field of releasing secondary dormancy of mulberry seeds, which can solve the problems of low germination rate and the like, and achieve the effects of improving germination rate, releasing dormancy and short treatment time.
Inactive Publication Date: 2016-07-20
NORTHEAST FORESTRY UNIVERSITY +2
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At present, in the test and production report of breaking mulberry seed dormancy, the effect of gibberellin is relatively obvious, but it can only increase the germination rate of mulberry seed to about 80% in a short period of time, and the germination rate is relatively low
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Embodiment 1-7
[0023] The present invention provides a method for releasing secondary dormancy of mulberry seeds, comprising the following steps:
[0024] Step 1, the mulberry seeds stored in the seed storage pit at 0-5°C are selected; mulberry seeds with a water content of 5% to 8%, a purity of 97% to 99%, and a purity higher than 99% are selected.
[0025] Step 2, soaking the seeds: soak the mulberry seeds in the concentration of 0mM, 20mM, 21mM, 22mM, 23mM, 24mM, 25mM CaCl 2 in the solution for 24 hours;
[0026] Step 3, tiling the seeds: place a double-layer filter paper in the petri dish, drop the same amount of sterile water on the filter paper until a water film is formed on the surface of the filter paper, take 0mM, 20mM, 21mM, 22mM, 23mM, 24mM, 25mM CaCl respectively 2 Seeds soaked in solution of 2 After the solution, 100 seeds with basically the same size and color were selected and plated on the petri dish; three biological replicates were performed.
[0027] Step 4, germinatio...
Embodiment 8-12
[0033] The present invention provides a method for releasing secondary dormancy of mulberry seeds, comprising the following steps:
[0034] Step 1, selection of mulberry seeds: mulberry seeds stored in 0-5 ℃ preservation cellar; select mulberry seeds with water content between 5% and 8%, purity between 97% and 99%, and clarity higher than 99% ;
[0035] Step 2, soaking the seeds: soak the mulberry seeds in a concentration of 25mM CaCl 2 in the solution for 24 hours;
[0036] Step 3, laying the seeds flat: place a double layer of filter paper in the petri dish, drop an equal amount of sterile water on the filter paper until a water film is formed on the surface of the filter paper, and wash the CaCl on the surface of the seeds with clean water 2 , choose 100 seeds with basically the same size and color, and spread them on the petri dish;
[0037] Step 4, germination culture: place the petri dish in an incubator with a relative humidity of about 80%, and set the temperature o...
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Abstract
The invention relates to the field of forest and grass planting, in particular to a method for breaking secondary dormancy of morus alba seeds. The method for breaking the secondary dormancy of the morus alba seeds includes steps of firstly, selecting the morus alba seeds; secondly, soaking the morus alba seeds in CaCl2 solution with the concentration of 20mM-25mM for 24 hours; thirdly, flatly laying the seeds, to be more specific, arranging double-layer filter paper in cultivation dishes, dropping an equal quantity of sterile water on the filter paper until water films are formed on the surfaces of the filter paper, washing CaCl2 on the surfaces of the seeds by the aid of deionized water and flatly laying 100 selected plump seeds with basically consistent sizes and colors on each cultivation dish; fourthly, germinating and cultivating the seeds, to be more specific, arranging the cultivation dishes in cultivation tanks with the relative humidity of approximately 80% and setting the temperatures of the cultivation tanks as 30-35 DEG C. The method for breaking the secondary dormancy of the morus alba seeds has the advantages that the method is easy to implement, the dormancy can be effectively broken, accordingly, the germination rate of the morus alba seeds can be increased, labor and materials can be saved, and morus alba can be advantageously popularized as a tree species with high economic value on a large scale.
Description
technical field [0001] The invention relates to the field of forest and grass planting, in particular to a method for releasing secondary dormancy of mulberry seeds. Background technique [0002] Mulberry is a small tree or shrub belonging to the Moraceae genus, dioecious. Mulberry trees are highly adaptable to both climate and soil. It is drought tolerant and moisture tolerant, and can also grow in warm and humid environments. It is most suitable for deep, loose and fertile soil, and can also withstand mild salinity, wind, smoke and dust, and can absorb nitrogen and sulfur oxides that pollute the atmosphere. The root system of mulberry is very developed, the growth rate is fast, the branches and leaves are resistant to pruning, the germination power is strong, and its lifespan is very long. Mulberry is one of the most important economic tree species in my country since ancient times. At present, the mulberry germplasm collected and preserved in my country belongs to 15 ...
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