A kind of seaweed spore aggregation seedling method

A technology of seaweed spores and aggregates, which is applied in seaweed cultivation, botany equipment and methods, applications, etc., can solve the problems that need to be further improved, cannot produce algae stably, and restrict the growth and development of new spores, so as to achieve strong practicability, The effect of short cycle time and easy operation

Active Publication Date: 2019-05-14
象山旭文海藻开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small size of the newly produced spores, and the uncontrollable factors that affect the survival rate of the spores such as water body, tide, season, water pollution, temperature, and salinity changes in the natural breeding sea area, the growth and development of the new spores are restricted, which will cause failure. Stable production of algae in large quantities ready for harvest
[0004] Although the main economic seaweeds have been artificially cultivated to varying degrees, the stability and quality of their yields still need to be further improved
At present, there is no relevant technology in China that uses the suspension growth of seaweed spore aggregates to form seedling breeding. Therefore, it is necessary to carry out the application of spore agglomeration method in seaweed seedling cultivation to obtain a method for seaweed seedling cultivation. This method can be used for artificial large-scale cultivation. Seaweed provides technical basis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of seaweed spore aggregation seedling method, comprises the following steps,

[0023] A. Take the algae growing in the natural state and cut them into fragments. After the cut algae are washed with sterilized seawater, they are placed in a light constant temperature incubator for cultivation. The temperature of the light constant temperature incubator is controlled at 20°C, and the photoperiod is controlled. In 12L:12D, the light intensity is controlled at 100μmol / m 2 / s, cultivated for 2 days to produce spores;

[0024] B. Collect the spore suspension produced by seaweed, concentrate until there are more than 6 spores in the field of view when monitored with an eyepiece 10×objective lens 40, and culture again; put the concentrated spore suspension into a light constant temperature incubator for cultivation, light light constant temperature incubator The temperature is controlled at 20°C, the photoperiod is controlled at 12L:12D, and the light intensity is contr...

Embodiment 2

[0027] A kind of seaweed spore aggregation seedling method, comprises the following steps,

[0028] A. Take the algae and cut them into fragments. After washing the cut algae with sterilized seawater, put them into a light constant temperature incubator for cultivation. The temperature of the light constant temperature incubator is controlled at 18°C, and the photoperiod is controlled at 12L:12D. The light intensity is controlled at 100μmol / m 2 / s, cultivated for 5 days to produce spores;

[0029] B. Collect the spore suspension produced by seaweed, concentrate until there are more than 6 spores in the field of view when monitored with an eyepiece 10×objective lens 40, and culture again; put the concentrated spore suspension into a light constant temperature incubator for cultivation, light light constant temperature incubator The temperature is controlled at 18°C, the photoperiod is controlled at 12L:12D, and the light intensity is controlled at 100μmol / m 2 / s, cultivated f...

Embodiment 3

[0033] A kind of seaweed spore aggregation seedling method, comprises the following steps,

[0034] A. Take the algae growing in the natural state and cut them into fragments. After washing the cut algae with sterilized seawater, put them in the light constant temperature incubator for cultivation. The temperature of the light constant temperature incubator is controlled at 25°C and the photoperiod is controlled. In 12L:12D, the light intensity is controlled at 100μmol / m 2 / s, cultivated for 3 days to produce spores;

[0035] B. Collect the spore suspension produced by seaweed, concentrate until there are more than 9 spores in the field of view when monitored with an eyepiece 10×objective lens 40, and cultivate again; put the concentrated spore suspension into a light constant temperature incubator for cultivation, light light constant temperature incubator The temperature is controlled at 25°C, the photoperiod is controlled at 12L:12D, and the light intensity is controlled a...

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PUM

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Abstract

The invention relates to the technical field of biology and discloses a spore aggregate seedling raising method for seaweed. According to the method, through carrying out external stimulus, such as cutting-off or high-temperature glare on the seaweed growing in a natural state, a large amount of spores can be generated simultaneously at a high speed. A generated spore suspension is collected, is concentration and then is subjected to culture under appropriate conditions. Under the culture conditions, the spores grow at the bottom of a glass dish at relatively high density and are aggregated to one another so as to form spore aggregates. One part of the spore aggregates are dispersed and then subjected to aerated re-culture so as to be developed into the seaweed, and the other part of the spore aggregates are subjected to low-temperature and low-light-intensity culture so as to preserve seeds. The provided method can provide a technical base for artificial formalized seaweed culture. The method has the major advantages that the spore aggregates grow in a nutrient solution in a suspended aggregation manner; the disadvantage in the traditional seedling raising that attachments and growth places are required to be provided for seaweed spore or zygotes and the like is avoided; and furthermore, the dispersed aggregates can be used for providing a guarantee of adjustable seedling quantity in subsequent further amplified culture.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for aggregating seaweed spores to grow seedlings. Background technique [0002] Our country has a vast sea area, and seaweed is an important resource in the ocean, such as many varieties of red algae, green algae and brown algae with very high economic value. However, in recent years, with the development of marine algae, the demand for algae has increased day by day, and the natural wild edible seaweed in sea areas has been far from meeting the demand for market quantity and quality. Therefore, expanding seaweed cultivation and processing technology is an important content for coastal countries. [0003] At present, the artificially cultivated seaweeds at home and abroad are mainly kelp, wakame, seaweed, Gracilaria, Eucheuma and reef film, etc. Most of them mainly adopt traditional seaweed cultivation methods. The traditional seaweed cultivation method is to use Protothec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01G33/00A01G33/02
CPCY02A40/80
Inventor 朱文荣朱萍
Owner 象山旭文海藻开发有限公司
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