Novel Mogrol derivative monomer
A technology of Mogrosin and its derivatives, applied in the field of medicine
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Embodiment 1
[0055] Example 1: Preparation of Mogrosanol Derivatives Monomers (3) and (4) from the Extract of 98% Mogrosin Total Saponins
[0056] Add 50g of Luo Han Guo extract (98% of the total saponins of Luo Han Guo, containing 55% of Mogroside V, purchased from Guilin Laiyin Biotechnology Co., Ltd.) to 250 mL of ethanol aqueous solution (the volume ratio of ethanol and water is 1:1) (prepared with Hydrochloric acid adjusted to a pH value of 3.0), stirred and dissolved in the reactor, then heated to 120 ° C for 2 hours, cooled to room temperature 25 ° C, the reaction solution was neutralized to a pH value of 7 with 1M NaOH lye, and in Distill the ethanol as much as possible at 90-95°C, add 200mL of distilled water to the remaining concentrated solution, stir thoroughly, cool and let it stand, separate and discard the upper water layer to obtain a brown extract; use 100mL, 50mL, and 50mL of acetic acid respectively Extract the brown extract with ethyl ester for 3 times, combine the 3 ex...
Embodiment 2
[0058] Example 2: Preparation of Mogrosanol Derivative Monomers (3) and (4) from the Extract of 95% Mogrosin Total Saponins
[0059] Add 50g of Luo Han Guo extract (95% of the total saponins of Luo Han Guo, containing 45% of mogroside V, purchased from Guilin Laiyin Biotechnology Co., Ltd.) into 250 mL of methanol aqueous solution (the volume ratio of methanol and water is 3:2) (pre-used Sulfuric acid is adjusted to a pH value of 2.8), stirred and dissolved in the reactor, and then heated to 120°C for 2 hours, cooled to room temperature, and the reaction solution is neutralized with 1M KOH lye to a pH value of 7. Distill the methanol as much as possible at 95°C, add 200mL of distilled water to the remaining concentrated solution, stir thoroughly, cool and let it stand, separate and discard the upper water layer to obtain a brown extract; use 100mL, 50mL, and 50mL ethyl acetate respectively Extract the coffee-colored extract 3 times, combine the 3 extracts, concentrate with a r...
Embodiment 3
[0060] Example 3: Preparation of mogrosanol derivative monomers (3) and (4) from the extract of 90% total saponins of Monk Fruit
[0061] Add 50g of Luo Han Guo extract (90% of Luo Han Guo total saponins, containing 40% mogroside V, purchased from Guilin Laiyin Biotechnology Co., Ltd.) into 250 mL of n-propanol aqueous solution (the volume ratio of n-propanol and water is 2:3) (Adjust the pH value to 3.2 with formic acid in advance, stir and dissolve in the reaction kettle, then heat up to 120 ° C for 2 hours, after cooling to room temperature, the reaction solution is neutralized with 1M NaOH lye to a pH value of 7, in Distill out n-propanol as much as possible at 90-95°C, add 200mL of distilled water to the remaining concentrated solution, stir thoroughly, cool and let it stand, separate and discard the upper water layer to obtain a brown extract; use 100mL, 50mL, Extract the brown extract with 50mL ethyl acetate for 3 times, combine the 3 extracts, concentrate with a rotary...
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