Method for promoting toadstool sclerotium formation efficiently

A technology of Morchella and sclerotia, applied in the field of efficiently promoting Morchella sclerotia formation, can solve problems such as large loss of active substances, complicated preparation operations, difficult to form a scientific and accurate method, and achieves easy operation and optimized hyphae. effect of growth

Active Publication Date: 2016-07-20
四川省食用菌研究所
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Problems solved by technology

Therefore, adding it to the medium can effectively accelerate the growth of hickory chick mycelium and the formation of sclerotia, but the traditional high temperature and moist heat sterilization will cause a relatively large loss of active substances, and it is not easy to control the dosage accurately in the whole process , it is not easy to form a scientific and accurate method
[0003] For example, Chinese patent application CN103937673A discloses a soil microbial culture medium and a preparation method thereof, but the method described in the patent still has certain defects: 1. The soil filtrate is sterilized by autoclaving, which cannot guarantee that the sterilized soil filtrate can Preserves all active ingredients that promote microbial growth
2. The preparation operation is more complicated, and the soil water bath and sedimentation take 3-7 hours in total, and the efficiency needs to be improved

Method used

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  • Method for promoting toadstool sclerotium formation efficiently
  • Method for promoting toadstool sclerotium formation efficiently
  • Method for promoting toadstool sclerotium formation efficiently

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] (1) Prepare 1L of PDA medium, sterilize with high temperature and damp heat, add 50mL of soil filtrate (1g / mL) filtered by a cell filter, add 25mL of medium improver, pour 20mL of improved PDA medium into each plate, place in ultra-clean Naturally solidified on the platform, as the test group;

[0067] (2) PDA medium is set as a control group;

[0068] (3) pick the mycelia of Morchella crassipes and inoculate them on the PDA medium and the improved PDA medium respectively;

[0069] (4) Culture in a fungal incubator (27°C);

[0070] (5) Observe the growth of hyphae and the appearance time of sclerotia.

[0071] Above-mentioned example result sees 1-3 and can be seen: the culture medium that has added PDA improver and Morchella stipe soil filtrate can effectively promote mycelia growth, and test group 6d can be covered with plate, is better than 8d of control group, simultaneously, bacteria The silk growth rate was also faster than that of the control group. The scler...

Embodiment 2

[0078] Experimental treatment According to Example 1, Morchella esculenta (Pers) Amans was used as inoculated mycelia to complete the experiment.

[0079] The results showed that the soil filtrate of the stipe of the morel stipe still had a promoting effect on the growth of the mycelium of the common morel. Compared with the traditional PDA medium, the growth rate of the common morel mycelium on the soil filtrate-improved medium was 28.76% faster , the formation time of sclerotia was 20% faster, and the number was 16.87% more. This experiment shows that the filtrate of the stipe soil of the morel stipe also has a promoting effect on the growth of the mycelia of the common morel and the formation of sclerotia.

Embodiment 3

[0081] Experimental treatment According to Example 1, Morchella importuna M. Kuoetal. was used as inoculated mycelia to complete the experiment.

[0082] The results showed that the soil filtrate of the stipe of the morel stipe also had a significant promoting effect on the growth of the mycelia of the morel stalk. The above experiments show that the soil filtrate of the selected morel stipe soil has a certain promotion effect on the growth of hickory chick mycelium and the formation of sclerotia, and the promotion effect is not limited to the hickory chick belonging to the same class in morphology bacteria. Explanation: The classification of morels and common morels belongs to the yellow morel family, and the ladder-shaped morel belongs to the black morel family.

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Abstract

The invention belongs to the field of fungus cultivation, and particularly relates to a method for promoting toadstool sclerotium formation efficiently. The method has the advantages that a soil suspension is filtered by a cell filter, and accordingly, ingredients beneficial to growth of toadstool mycelia in soil are preserved effectively; a PDA (potato dextrose agar) culture medium modifier is adopted, so that growth of the toadstool mycelia is optimized and substantial sclerotiums are generated quickly. The method includes: A, mixing high-organic-content soil with toadstool stipe soil; B, adding water, mixing evenly, centrifuging, taking supernate, and filtering the supernate to obtain soil filtrate; C, adding the soil filtrate obtained in the step B and the culture medium modifier into a PAD culture medium, and mixing evenly to obtain an efficient toadstool culture medium, putting the efficient toadstool culture medium in a culture utensil, and cooling and solidifying the efficient toadstool culture medium for standby application; D, inoculating the efficient toadstool culture medium with the toadstool mycelia, and culturing the toadstool mycelia according to normal culture conditions to obtain the sclerotiums.

Description

technical field [0001] The invention belongs to the field of fungal cultivation, and in particular relates to a method for efficiently promoting the formation of morel sclerotia. Background technique [0002] Morel (Morchella) meat is delicious and crisp, with high nutritional and medicinal value. For a long time, people have been committed to the artificial cultivation of Morchella, but its complex life cycle and various environmental factors have brought great difficulties to the realization of this goal. After research, many scientists believe that the sclerotium is an important stage in the life cycle of the Morchella fungus, and only when the sclerotia grows can it be possible to obtain the ascomycetes. Therefore, how to accelerate the growth of hickory chick hyphae and grow sclerotium fast is a problem worth considering. Methods such as improving medium and optimizing culture conditions have been used to accelerate the growth of morel mycelium, and achieved certain r...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 陈影彭卫红甘炳成唐杰李小林熊川黄忠乾王勇谢丽源何晓兰刘理旭闵江姜邻
Owner 四川省食用菌研究所
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