Strain preparation method of black skin collybia albuminosa stock strain

A technology of Gallatella vulgaris and original seed, applied in the fields of botanical equipment and methods, fungi, horticulture, etc., can solve the problems of unscientific medium composition and poor quality of original seed, etc., to improve the quality, enhance the quality of mother seed, The effect of promoting the growth of mycelium

Inactive Publication Date: 2018-03-16
四川山水美地农业投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the problems existing in the prior art, the object of the present invention is to provide a kind of scientific optimization of the components of the culture medium in the original seed culture process, thereby improving the seed production method of the quality of the original seed of P. Problems of Unscientific Medium Components and Poor Quality of the Produced Original Seeds During the Cultivation Process of the Original Species of Gallinarum nigricans

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of seed production method of the original species of P. nigella, comprising the following steps:

[0029] Step 1): mother seed preparation, specifically including:

[0030] Step 1.1): Under aseptic conditions, the fruiting bodies of Gallus pilosulae are separated to obtain fruiting body mycelium;

[0031] Step 1.2): Separating the rhizoids of Gallinarum nigricans into tissues to obtain rhizoid mycelia;

[0032] Step 1.3): transfer the fruiting body mycelium to a test tube filled with liquid medium, cultivate at 25°C, insert the rhizoid mycelium when the mycelium grows to 1.3-1.8cm, and continue culturing for 6 days to obtain mature mother species;

[0033] Step 2): Preparing the original seed, inserting the mature female seed prepared in step 1) into the original seed medium, culturing for 22 days to mature, and obtaining the original seed;

[0034] Among them, the composition and mass ratio of the original seed medium are: 50 parts of cottonseed hulls, 15 par...

Embodiment 2

[0039] A kind of seed production method of the original species of P. nigella, comprising the following steps:

[0040] Step 1): mother seed preparation, specifically including:

[0041] Step 1.1): Under aseptic conditions, the fruiting bodies of Gallus pilosulae are separated to obtain fruiting body mycelium;

[0042] Step 1.2): Separating the rhizoids of Gallinarum nigricans into tissues to obtain rhizoid mycelia;

[0043] Step 1.3): transfer the fruiting body mycelium to a test tube filled with liquid medium, cultivate at 22°C, insert the rhizoid mycelium when the mycelium grows to 1.3-1.8 cm, and continue culturing for 8 days to obtain mature mother species;

[0044] Step 2): Preparing the original seed, inserting the mature female seed prepared in step 1) into the original seed medium, culturing for 25 days to mature, and obtaining the original seed;

[0045] Among them, the composition and mass ratio of the original seed medium are: 50 parts of wood chips, 3 parts of ...

Embodiment 3

[0050] A kind of seed production method of the original species of P. nigella, comprising the following steps:

[0051] Step 1): mother seed preparation, specifically including:

[0052] Step 1.1): Under aseptic conditions, the fruiting bodies of Gallus pilosulae are separated to obtain fruiting body mycelium;

[0053] Step 1.2): Separating the rhizoids of Gallinarum nigricans into tissues to obtain rhizoid mycelia;

[0054] Step 1.3): Transfer the fruiting body mycelium to a test tube filled with liquid medium, culture at 24°C, insert the rhizoid mycelium when the mycelium grows to 1.3-1.8cm, and continue culturing for 6 days to obtain mature mother species;

[0055] Step 2): Preparing the original seed, inserting the mature female seed prepared in step 1) into the original seed medium, culturing for 24 days to mature, and obtaining the original seed;

[0056] The composition and mass ratio of the original seed culture medium are: 50 parts of corn cob, 5 parts of wheat flo...

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PUM

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Abstract

The invention discloses a strain preparation method of a black skin collybia albuminosa stock strain. The method comprises the steps of 1, mother strain preparation, wherein sporocarp mycelium is inoculated to a test tube filled with a liquid culture medium to be cultured, rhizoid mycelium is inoculated, and culturing is conducted to obtain the mature mother strain; 2, stock strain preparation, wherein the mother strain is inoculated into a stock strain culture medium, and culturing is conducted to prepare the stock strain. Through combined culturing of the sporocarp mycelium and the rhizoid mycelium, the mother strain is prepared, and through combined culturing of the two kinds of mycelium, the hypha growth can be effectively promoted, and the quality of the mother strain is strengthened.Plant stem powder, curare grain powder and saccharose are adopted by the stock strain culture medium to provide a carbon source and energy substances for the growth of hpyhae, metal salts provide essential metal ions for the growth and development of the hyphae, and acid compounds provide an essential acid environment for the growth and development of the hyphae to promote the growth of the hyphae; guaiacol provides signals for laccase secretion in the growth and development process of the hyphae, and water provides essential moisture and maintains essential humidity for the growth and development of the hyphae.

Description

technical field [0001] The invention belongs to the technical field of chicken fungus cultivation, and in particular relates to a seed production method of the original species of Gallibicus nigricans. Background technique [0002] It is one of the famous wild edible mushrooms, the taste of which is especially the best. Traditional Chinese medicine believes that the fungus is flat and sweet, has the effects of invigorating the spleen and Qi, appetizing and refreshing, and relieving pain and edema. It is an ideal food for treating hemorrhoids. Modern medicine has discovered that in addition to having special effects on hemorrhoids, chicken fir can also prevent colon cancer, lower blood pressure, and enhance human immunity. However, simply picking wild chicken fir is difficult to meet the huge market demand, and people began to cultivate it artificially. However, due to the close relationship between the growth and development of P. nigricans and the activities of soil termit...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20
CPCC12N1/14
Inventor 吴玲周善龙
Owner 四川山水美地农业投资有限公司
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