Detection method for aflatoxin in Chinese herbal pieces
A technology of aflatoxin and traditional Chinese medicine decoction pieces, which is applied in the field of analytical chemistry, can solve the problems of difficulty in breaking the wall of fungi, unfavorable detection personnel to accurately judge the results, and low efficiency of high performance liquid chromatography fluorescence detection methods, so as to achieve high efficiency and improve accuracy Effect
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Embodiment 1
[0018] A kind of detection method of aflatoxin in Chinese medicine decoction pieces, comprises the following steps:
[0019] (1) Add Chinese herbal decoction pieces into a pulverizer with a power of 1.3kW / h, a rotating speed of 2800 rpm, and an hourly input volume of 2kg of decoction pieces, and pulverize and grind them into powder, with a particle size of 200 mesh;
[0020] (2) Soak the powder obtained in step (1) in acetic acid with a mass concentration of 0.5%% for 0.5 hours, rinse with distilled water until neutral, and dry in an oven at 56°C;
[0021] (3) Extract the powder dried in step (2) with acetonitrile with a mass concentration of 78%, shake the extract for 10 minutes, centrifuge at 4°C and 6000 rpm for 5 minutes, and take the supernatant;
[0022] (4) Adopt the supernatant in high performance liquid chromatography detection step (3), mobile phase is the ammonium acetate of 3mol / L and the acetic acid of 0.1% mix by volume ratio 1:1, flow velocity is 0.3mL / min, colu...
Embodiment 2
[0023] A kind of detection method of aflatoxin in Chinese medicine decoction pieces, comprises the following steps:
[0024] (1) Add Chinese herbal decoction pieces into a pulverizer with a power of 1.5kW / h, a rotating speed of 3000 rpm, and an hourly input volume of 3.5kg of decoction pieces, and pulverize and grind them into powder with a particle size of 300 mesh;
[0025] (2) Soak the powder obtained in step (1) in acetic acid with a mass concentration of 1.7% for 2 hours, rinse with distilled water until neutral, and dry in an oven at 69°C;
[0026] (3) Extract the powder dried in step (2) with acetonitrile with a mass concentration of 81%, shake the extract for 20 minutes, centrifuge at 4°C and 7000 rpm for 8 minutes, and take the supernatant;
[0027] (4) adopt high-performance liquid chromatography to detect the supernatant in the step (3), the ammonium acetate of mobile phase is 4mol / L and the acetic acid of 0.2% is mixed by volume ratio 2:1, flow velocity is 0.6mL / mi...
Embodiment 3
[0028] A kind of detection method of aflatoxin in Chinese medicine decoction pieces, comprises the following steps:
[0029] (1) Add Chinese herbal decoction pieces into a pulverizer with a power of 1.7kW / h, a rotating speed of 3200 rpm, and an hourly input volume of 5kg of decoction pieces, and pulverize and grind them into powder with a particle size of 400 mesh;
[0030] (2) Soak the powder obtained in step (1) in acetic acid with a mass concentration of 2.5% for 3 hours, rinse with distilled water until neutral, and dry in an oven at 78°C;
[0031] (3) Extract the powder dried in step (2) with acetonitrile with a mass concentration of 84%, shake the extract for 25 minutes, centrifuge at 4°C and 8000 rpm for 10 minutes, and take the supernatant;
[0032] (4) Adopt the supernatant in the high performance liquid chromatography detection step (3), the ammonium acetate of mobile phase is 5mol / L and the acetic acid of 0.3% mixes by volume ratio 3:1, flow velocity is 0.8mL / min, c...
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