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A preparation method of honeycomb silk fibroin porous microsphere drug slow-release carrier

A technology of silk fibroin and porous microspheres, which is used in pharmaceutical formulations, drug combinations, and medical preparations containing active ingredients, etc., to achieve the effects of excellent biocompatibility, high biosafety, and easy operation.

Active Publication Date: 2018-11-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of silk fibroin to regulate the application of hydroxyapatite in the field of sustained drug release is still little research

Method used

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  • A preparation method of honeycomb silk fibroin porous microsphere drug slow-release carrier
  • A preparation method of honeycomb silk fibroin porous microsphere drug slow-release carrier
  • A preparation method of honeycomb silk fibroin porous microsphere drug slow-release carrier

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Experimental program
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Embodiment 1

[0036] In this embodiment, the preparation method of the honeycomb silk fibroin porous microsphere drug slow-release carrier includes the following steps in turn:

[0037] (1) dissolving the fibrous silk fibroin formed after silkworm cocoon degumming, and obtaining an aqueous silk fibroin protein solution after filtration, dialysis, and concentration;

[0038] (2) Adjust the concentration of the silk fibroin solution in step (1) to 0.1 mg / mL and disperse it by ultrasound, put it into a dialysis bag and soak it in different times of human simulated body fluid. : 100 rpm.

[0039] (3) The shaker treatment time is 14 days, and the simulated body fluid in step (2) is replaced every 24 hours to obtain the microsphere suspension;

[0040] (4) The microsphere suspension in step (3) was washed several times with deionized water and absolute ethanol, centrifuged, and freeze-dried for 5 hours to obtain apatite / silk fibroin microspheres with a diameter of 1-5 μm. ;

[0041] (7) The bi...

Embodiment 2

[0043] The biomineralization method of the tussah silk fibroin that promotes cell growth in the present embodiment comprises the following steps successively:

[0044] (1) dissolving the fibrous silk fibroin formed after silkworm cocoon degumming, and obtaining an aqueous silk fibroin protein solution after filtration, dialysis, and concentration;

[0045] (2) Adjust the concentration of the silk fibroin solution in step (1) to 50 mg / mL and disperse it by ultrasonic waves, put it into a dialysis bag and soak it in different times of human simulated body fluid, the solution temperature is 37°C, and the rotation speed is: 200 rpm.

[0046] (3) The shaker treatment time is 1 day, and the simulated body fluid in step (2) is replaced every 24 hours to obtain the microsphere suspension;

[0047] (4) The microsphere suspension in step (3) was washed several times with deionized water and absolute ethanol, centrifuged, and dried at 60°C for 10 hours to obtain apatite / silk fibroin mic...

Embodiment 3

[0053] The biomineralization method of the tussah silk fibroin that promotes cell growth in the present embodiment comprises the following steps successively:

[0054] (1) dissolving the fibrous silk fibroin formed after silkworm cocoon degumming, and obtaining an aqueous tussah silk fibroin protein solution after filtration, dialysis and concentration;

[0055] (2) Adjust the concentration of the tussah silk fibroin protein solution in step (1) to 2 mg / mL and disperse it ultrasonically, put it into a dialysis bag and soak it in 5 times the simulated body fluid of the human body, the solution temperature is 37 °C, and the rotation speed : 20 rpm.

[0056] (3) The shaker treatment time is 7 days, and the simulated body fluid in step (2) is replaced every 24 hours to obtain the microsphere suspension;

[0057] (4) The microsphere suspension in step (3) was washed several times with deionized water and absolute ethanol, centrifuged, and freeze-dried for 48 hours to obtain apatit...

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Abstract

The invention discloses a method for preparing a honeycomb silk fibroin porous microsphere sustained drug release vector. Fibrous fibroin formed after cocoon degumming is dissolved, filtered, dialyzed and concentrated to obtain silk fibroin aqueous solution; the silk fibroin aqueous solution is subject to ultrasonic dispersion, placed in a dialysis bag and placed in simulated human body fluids of different times concentrations to be soaked, the silk fibroin aqueous solution is processed in a uniform shaking table, the simulated human body fluids are replaced at time intervals to obtain microsphere suspension; and the microsphere suspension is washed many times and undergoes centrifugal separation and drying to obtain apatite / silk fibroin microsphere powder as the sustained drug release vector. Apatite microspheres of a certain size and pore diameters can be formed on the surface of silk fibroin, the dispersity, drug loading performance and sustained release performance are excellent, the honeycomb silk fibroin porous microsphere sustained drug release vector has no toxic or side effect on normal cells, the biocompatibility of the drug vector is improved remarkably, and the honeycomb silk fibroin porous microsphere sustained drug release vector has wide application prospects in the fields such as sustained drug release, tissue engineering and enzyme engineering.

Description

technical field [0001] The invention discloses a preparation method of a honeycomb silk fibroin porous microsphere drug slow-release carrier, which belongs to the field of biomedical materials. Background technique [0002] Silk fiber is one of the earliest natural proteins used by humans. The natural protein fiber mainly composed of silk fibroin is a biodegradable biopolymer material with excellent biocompatibility, mechanical properties and low immunogenicity. Ideal material for the preparation of biomaterials. In recent years, with the rapid development of silk protein materials in biomedical technology, they have been widely used in many fields such as health care products, biomedicine, tissue engineering, and drug sustained release. . [0003] The role of drug slow-release carrier is to provide continuous drug delivery to the site of administration (such as: tumor tissue), and the drug sustained release for a long time (for example, several days or weeks) provides the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/19A61K47/42A61K47/02A61K31/704A61P35/00
CPCA61K9/19A61K31/704A61K47/02A61K47/42
Inventor 杨明英帅亚俊毛传斌
Owner ZHEJIANG UNIV
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