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Mother strain culture medium capable of preventing degeneration of agaricus bisporus strain and preparation method thereof

A technology of mother seed culture medium and strain degradation, which is applied to fertilizers made from biological waste, application, organic fertilizers, etc., can solve the problems of long cultivation time, strain degradation, slow growth of Agaricus bisporus mycelium, etc., to ensure that the bacteria The quality, the effect of solving the degradation problem

Inactive Publication Date: 2016-08-10
中国农业大学烟台研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Agaricus bisporus enterprises mostly use potato dextrose agar medium (PDA medium) for the cultivation of Agaricus bisporus mother species. This medium is suitable for the cultivation of most fungi, and the cost is low. Strain degradation will occur, which will affect the quality of Agaricus bisporus parent species

Method used

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  • Mother strain culture medium capable of preventing degeneration of agaricus bisporus strain and preparation method thereof

Examples

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Effect test

Embodiment 1

[0019] A mother seed culture medium capable of preventing the degradation of Agaricus bisporus strains comprises the following components in parts by weight: 100g of potatoes, 20g of cottonseed hulls, 20g of agar, and 1000ml of water.

[0020] The preparation method of above-mentioned mother seed culture medium is as follows:

[0021] 1) Peel the potatoes and cut into pieces;

[0022] 2) Weigh 100g of potatoes and 20g of cottonseed husks, put them in 1000ml of water, heat and boil for 30min, filter with 4 layers of gauze;

[0023] 3) Add 20 grams of agar to the obtained filtrate, heat and melt;

[0024] 4) Divide into test tubes, bandage, and sterilize at 120°C for 20 minutes to obtain the product.

Embodiment 2

[0026] A kind of mother culture medium that can prevent the degradation of Agaricus bisporus strains, comprising the following components by weight: 140g of potatoes, 30g of cottonseed hulls, 3mg of manganese sulfate, 3mg of magnesium sulfate, 3ug of ferrous sulfate, 1 mg of potassium dihydrogen phosphate gram, glucose 10 grams, agar 20 grams, water 1000ml.

[0027] The preparation method of above-mentioned mother seed culture medium is as follows:

[0028] 1) Peel the potatoes and cut into pieces;

[0029] 2) Weigh 140g of potatoes and 30g of cottonseed hulls, place them in 1000ml of water, heat and boil for 30min, filter with 4 layers of gauze;

[0030] 3) Add 3 mg of manganese sulfate, 3 mg of magnesium sulfate, 3 ug of ferrous sulfate, 1 gram of potassium dihydrogen phosphate, and 10 grams of glucose to the obtained filtrate to dissolve;

[0031] 4) Add 20 grams of agar to the resulting filtrate, heat and melt;

[0032] 5) Divide into Erlenmeyer flasks, bandage, and ste...

Embodiment 3

[0034] A kind of mother seed culture medium that can prevent Agaricus bisporus from degenerating, comprises the following components by weight: 180g of potatoes, 40g of cottonseed hulls, 5mg of manganese sulfate, 5mg of magnesium sulfate, 5ug of ferrous sulfate, potassium dihydrogen phosphate 2 gram, glucose 20 grams, agar 20 grams, water 1000ml.

[0035] The preparation method of above-mentioned mother seed culture medium is as follows:

[0036] 1) Peel the potatoes and cut into pieces;

[0037] 2) Weigh 180g of potatoes and 40g of cottonseed husks, place them in 1000ml of water, heat and boil for 30min, filter with 4 layers of gauze;

[0038] 3) Add manganese sulfate 5mg, magnesium sulfate 5mg, ferrous sulfate 5ug, potassium dihydrogen phosphate 2 grams, glucose 20 grams to dissolve in gained filtrate;

[0039] 4) Add 20 grams of agar to the resulting filtrate, heat and melt;

[0040] 5) Divide into Erlenmeyer flasks, bandage, and sterilize at 120°C for 20 minutes to obta...

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Abstract

The invention relates to a mother strain culture medium capable of preventing degeneration of an agaricus bisporus strain; the mother strain culture medium comprises the following components in parts by weight: 100-180 parts of potato, 20-60 parts of cottonseed hulls, 0-0.005 part of manganese sulfate, 0-5*10<-3> part of magnesium sulfate, 0-5*10<-6> part of ferrous sulfate, 0-2 parts of monopotassium phosphate, 0-20 parts of glucose, 20 parts of agar, and 1000 parts of water. The culture medium has the beneficial effects that the agaricus bisporu mother strain culture medium prepared by the formula and the method is used for culturing the agaricus bisporus mother strain for a long time (more than or equal to 30 days) and has no strain degeneration phenomenon found out, effectively solves the degeneration problem of the agaricus bisporus mother strain, and guarantees the quality of the strain.

Description

technical field [0001] The invention relates to a Agaricus bisporus culture medium and a preparation method thereof, in particular to a Agaricus bisporus stock culture medium and a preparation method thereof, belonging to the technical field of edible fungus cultivation. Background technique [0002] Agaricus bisporus (Agaricus bisporus), also known as mushrooms, white mushrooms, mushrooms, etc., belongs to the Basidiomycotina, Phytomycota, Agaricaceae Agaricaceae, and Mushroom genus in the taxonomy of fungi. At present, more than 100 countries and regions in the world cultivate Agaricus bisporus, which is the edible fungus with the highest output and largest consumption in the world. [0003] The production process of Agaricus bisporus is as follows: After the strain grows well on the mother seed medium, it is inoculated into the original seed medium to produce the original seed, after the original seed grows well, it is inoculated into the cultivated medium to produce the ...

Claims

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Application Information

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IPC IPC(8): C05G3/00
CPCC05B7/00C05G3/00C05D5/00C05D9/02C05F5/002C05F11/00
Inventor 王鸿磊王红艳丁强李梅崔从光邹积华
Owner 中国农业大学烟台研究院
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