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Microscopic ornamental plant and culture method thereof

A technology for ornamental plants and cultivation methods, applied in the fields of botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of cumbersome maintenance, slow plant growth, shortfall in success, etc. improved effect

Inactive Publication Date: 2016-08-31
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And under normal circumstances, the growth of plants is slow, and the initial maintenance is relatively cumbersome, and a little carelessness may fall short

Method used

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  • Microscopic ornamental plant and culture method thereof
  • Microscopic ornamental plant and culture method thereof
  • Microscopic ornamental plant and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Prepare the medium according to the following formula: MS 4.4g / L, sucrose 30g / L, gellan gum 3g / L, 6-BA 1.0mg / L, IAA 1.0mg / L, melanin and brilliant blue 300μL / L;

[0021] Put the culture medium in a glass bottle with a cover, inoculate the sterilized succulent plant Hongzhiyu leaves as explants into the above culture medium, and place it in the tissue culture room. The temperature of the tissue culture room is 25°C, and the light The time is 24h / d, the humidity is 60%, and the light intensity is 1300Lx. After cultivating for 15 days, 84.3% of the rainbow jade takes root and takes root in the medium to grow, and the plants of the rainbow jade are obtained, and their growth conditions are good. See figure 1 .

[0022] Taking the culture medium without pigment as the control group, 53.5% of the rainbow jade roots are aerial roots, and a few roots grow in the medium, see figure 2 .

Embodiment 2

[0024] Prepare the medium according to the following formula: MS2.2g / L, sucrose 20g / L, gellan gum 2g / L, 6-BA 0.5mg / L, IAA 0.5mg / L, amaranth, carmine, allura 300μL / L;

[0025] Put the culture medium in a glass bottle with a cover, inoculate the sterilized succulent plant Hongzhiyu leaves as explants into the above culture medium, and place it in the tissue culture room. The temperature of the tissue culture room is 25°C, and the light The time is 24h / d, the humidity is 60%, and the light intensity is 1300Lx. After 15 days of cultivation, the plants of Rainbow Jade are obtained.

Embodiment 3

[0027] The formulation and method of this example are the same as those of Example 2, only the pigment is different. The pigment of this example is carmine 200 μL / L. After 15 days of cultivation, the plants of rainbow jade are obtained.

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Abstract

The invention relates to a microscopic ornamental plant and a culture method thereof, belonging to the technical field of plant culture. The main technical scheme provided by the invention is as follows: the culture method comprises the following steps: firstly, sterilizing a plant explant; secondly, grafting the sterilized plant explant into a bottle filled with a culture medium, and culturing for 10 to 60 days to obtain the microscopic ornamental plant, wherein the culture medium is mainly prepared from the following raw materials: 2.2 to 4.4g / L of MS, 20 to 30g / L of sucrose, 2 to 7g / L of gellan gum, 0.5 to 5.0gm / L of 6-BA and 10 to 400mu L / L of pigment. According to the culture method provided by the invention, the influence from an outside natural environment is avoided; the culture medium can be a single-color culture medium or a colorful multilayer culture medium; the rooting percentage of the plants in the culture medium in which the pigment is added is greatly improved, so that the survival rate of plants is accordingly improved.

Description

technical field [0001] The invention relates to a method for cultivating plants, in particular to a method for cultivating miniature ornamental plants, and belongs to the technical field of plant cultivation. Background technique [0002] Along with improving constantly of people's living standard, indoor ornamental plants are also more and more subject to people's favor. At present, the cultivation of indoor ornamental plants is mainly container cultivation, and the commonly used containers for container cultivation are pottery or plastic pots; the cultivation substrate is generally soil or soilless substrate. Moreover, the culture medium of tissue culture seedlings has no special color. Because different plants have different requirements on the physical and chemical properties of the substrate, and the shape of the container directly affects the physical and chemical properties of the substrate. Soil is a commonly used substrate in ornamental plant cultivation. Under na...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 金华刘硕郭鹏邹吉祥李琳琳盛莉
Owner DALIAN NATIONALITIES UNIVERSITY