Application of Linoleoylethanolamine in Improving Plant Resistance to Yellow Leaf Curl Virus Disease
A technology of linoleoylethanolamine and yellow leaf curl virus, which is applied in the fields of application, plant growth regulator, plant growth regulator, etc., can solve the problems of less research and application, so as to extend the validity period, improve drug efficacy, and improve resistance The effect of rainwater wash-off capacity
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Embodiment 1
[0050] 1. Preparation of preparations
[0051] Get 32.3mL of 50mg / mL ethanol solution of linoleoylethanolamine (LEA) (the drug is purchased from SIGMA); then slowly add 57.7mL of water, and mix the mixed solution evenly, and finally add 10.0mL of organic The silicon solution is uniformly stirred to obtain a preparation.
[0052] 2. Application and testing of preparations
[0053] Take 20 ml of the above preparation, add 10 liters of water, mix well, and spray evenly on the surface of tomato leaves with four leaves and one heart at the seedling age in the evening until the leaves are wet, and continue spraying for 2 days. control.
[0054] The above-mentioned treated plants were picked with sterilized bamboo sticks to pick up the invasive clones of Agrobacterium and inoculated with tomato yellow leaf curl virus by acupuncture at the stalk of the first to second true leaf of tomato. All the above-treated plants were placed at 28°C, 70% humidity, 12h light / 12h dark, and the li...
Embodiment 2
[0059] 1. Preparation of preparations
[0060] Get 50mg / mL of linoleoylethanolamine (LEA) ethanol solution 25.9mL (medicine purchased from SIGMA company); then slowly add 61.6mL of water, and mix the mixed solution evenly, finally add 12.5mL of organic The silicon solution is uniformly stirred to obtain a preparation.
[0061] 2. Application and testing of preparations
[0062] Take 20 ml of the above preparation, add 10 liters of water, mix well, and spray evenly on the surface of tomato leaves with three leaves and one heart at the seedling age in the evening until the leaves are wet, and continue spraying for 2 days. control.
[0063] The above-mentioned treated plants were picked with sterilized bamboo sticks to pick up the invasive clones of Agrobacterium and inoculated with tomato yellow leaf curl virus by acupuncture at the stalk of the first to second true leaf of tomato. All the plants treated above were placed at 32°C, 70% humidity, 12h light / 12h dark, and the lig...
Embodiment 3
[0068] 1. Preparation of preparations
[0069] Take 42.1 mL of 50 mg / mL ethanol solution of linoleoylethanolamine (LEA) (purchased from SIGMA); slowly add 42.9 mL of water, mix the mixed solution evenly, and finally add 15 mL of organic silicon The solution is uniformly stirred to obtain a preparation.
[0070] 2. Application and testing of preparations
[0071] Select two adjacent plastic greenhouses with the same cultivation technology and management, and use the above-mentioned preparation on the treatment shed during the high temperature and less rainy season in summer. Take 40 ml of the preparation, add 20 liters of clear water, mix well, and divide it twice in the morning and evening. Spray evenly on the surface of tomato leaves with a seedling age of seven leaves and one heart until the leaves are wet, and continue spraying for 2 days; while the control shed only sprays the same volume of water.
[0072] After 20 days, the disease incidence of 60 tomato plants in the ...
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