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A method for inducing bone marrow mesenchymal stem cells to differentiate into liver-like cells

A technology for bone marrow mesenchymal and hepatocyte-like cells is applied in the field of inducing the differentiation of bone marrow mesenchymal stem cells into hepatoid cells, which can solve the problems of not optimal physiological environment, incomplete liver-like cells with hepatocytes, etc., and achieve minimal impact. , the effect of easy operation

Active Publication Date: 2018-06-22
XINXIANG MEDICAL UNIV
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  • Abstract
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Problems solved by technology

[0003] In the prior art, the method of inducing bone marrow mesenchymal stem cells to differentiate into liver cells, because the induced physiological environment is not optimal, so the induced hepatic cells do not fully have the characteristics of hepatocytes

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  • A method for inducing bone marrow mesenchymal stem cells to differentiate into liver-like cells
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  • A method for inducing bone marrow mesenchymal stem cells to differentiate into liver-like cells

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Embodiment Construction

[0016] The purpose of the present invention is to find a suitable source of hepatocytes required for hepatocyte transplantation, and to study the effects of different induction methods on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into hepatocyte-like cells in vitro.

[0017] Materials used in the present invention, L-DMEM medium is purchased from U.S. Gibco Company, fetal bovine serum is purchased from U.S. Hyclone Company, trypsin is purchased from U.S. Sigma Company, albumin (Albumin, ALB) and alpha-fetoprotein (α-fetoprotein, FP or AFP) monoclonal antibody was purchased from Santa Cruz Company in the United States, SD rats were purchased from the Experimental Animal Center of Xinxiang Medical College, clean grade, and the animals were handled in accordance with animal ethics standards during the experiment.

[0018] The present invention sets up four groups of experiments respectively, group A uses only fetal bovine serum (FBS, fetal bovine serum) as ...

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Abstract

The invention provides a method for inducing differentiation of mesenchymal stem cells into liver-like cells. The method comprises the following steps: separating and culturing mesenchymal stem cells, collecting serum subjected to liver regeneration for 12 hours, taking liverregenerated for 12 hours to prepare liver tissue homogenate, and culturing the mesenchymal stem cells inductively in the serum and the liver tissue homogenate. The liver-like cells induced by the method for inducing the differentiation of mesenchymal stem cells into liver-like cells completely have the characteristics of the liver cells.

Description

technical field [0001] The invention relates to the field of cell and molecular biology, in particular to a method for inducing bone marrow mesenchymal stem cells to differentiate into liver-like cells. Background technique [0002] Liver transplantation is currently an effective method for the treatment of various end-stage liver diseases. However, due to factors such as limited donor sources, high cost, and immune rejection, it is difficult to promote in large quantities, thus limiting the clinical application of liver transplantation. With the rise and development of tissue engineering, cell replacement therapy will become a new way to overcome some difficult diseases. Bone marrow mesenchymal stem cells (BMSCs) have strong proliferation ability, strong multi-directional differentiation potential, low immune rejection, wide range of sources, easy to obtain materials, less damage, no ethical problems, and good tissue compatibility, it is expected to become a new type of se...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N5/0775
CPCC12N5/067C12N2506/1353
Inventor 邢雪琨王树芳张艳芬李永海陈红丽徐志浩南文滨林俊堂丰慧根袁志庆
Owner XINXIANG MEDICAL UNIV