Preparation of immobilized enzyme and method for hydrolyzing diosgenin

A technology of diosgenin and immobilized enzymes, applied in the field of biomedicine, can solve problems such as environmental pollution, achieve the effects of reducing production costs, increasing conversion rates, and mild reaction conditions

Active Publication Date: 2020-09-01
华慧海洋多糖生物技术(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the processing of diosgenin needs to use a large amount of hydrochloric acid to carry out high-temperature pressurized hydrolysis of diosgenin in turmeric. During the hydrolysis process, the starch in turmeric, fiber and the like can be partially or completely hydrolyzed, and the resulting sugar liquid and The remaining acid has caused serious pollution to the environment

Method used

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  • Preparation of immobilized enzyme and method for hydrolyzing diosgenin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) 100kg of dry turmeric raw materials are pulverized, passed through a 20-mesh sieve, added to 300kg, and 20000IU of raw material amylase, 1000IU of pullulanase, and 100000IU of fungal α-amylase are added to the above-mentioned solution for enzymolysis;

[0037] (2) enzymatically hydrolyzing the turmeric aqueous solution in step (1) for 24h at natural temperature;

[0038] (3) Step (2) is subjected to solid-liquid separation, and the solid is extracted with 100 L of ethanol with a volume fraction of 75% to extract dioscin, extracting 3 times, each time for 30 minutes, and the liquid can be used to make alcohol;

[0039] (4) Preparation of immobilized enzyme preparation composition:

[0040] Add water to chitosan to prepare a solution with a concentration of 2g / L, stir it at a temperature of 10°C to dissolve it; then add β-glucosidase 12000IU and cascara with a mass ratio of 1:30 to chitosan Glycosidase 2000IU, stir evenly, place on a constant temperature shaker at 10...

Embodiment 2

[0044] (1) 100kg of dried turmeric raw materials are crushed, passed through a 20-mesh sieve, added to 300kg, 10000IU of raw material amylase, 5000IU of pullulanase, and 50000IU of fungal α-amylase are added to the above-mentioned solution for enzymolysis;

[0045] (2) enzymatically hydrolyzing the turmeric aqueous solution in step (1) for 24h at natural temperature;

[0046] (3) Step (2) is subjected to solid-liquid separation, and the solid is extracted with 100 L of ethanol with a volume fraction of 75% to extract dioscin, extracting 3 times, each time for 30 minutes, and the liquid can be used to make alcohol;

[0047] (4) Preparation of immobilized enzyme preparation composition:

[0048]Mix chitosan and non-polar macroporous resin at 1:1, add water to prepare a solution with a concentration of 2g / L, stir at a temperature of 10°C to dissolve it; then add chitosan with a mass ratio of 1:40 β-glucosidase 8000IU and rhamnosidase 5000IU, stir evenly, place on a constant temp...

Embodiment 3

[0052] (1) 100kg of dried turmeric raw materials are pulverized, passed through a 20-mesh sieve, added to 300kg, and 16000IU of raw material amylase, 2000IU of pullulanase, and 90000IU of fungal α-amylase are added to the above-mentioned solution for enzymolysis;

[0053] (2) enzymatically hydrolyzing the turmeric aqueous solution in step (1) for 24h at natural temperature;

[0054] (3) Step (2) is subjected to solid-liquid separation, and the solid is extracted with 100 L of ethanol with a volume fraction of 75% to extract dioscin, extracting 3 times, each time for 30 minutes, and the liquid can be used to make alcohol;

[0055] (4) Preparation of immobilized enzyme preparation composition:

[0056] Mix chitosan and non-polar macroporous resin polyacrylamide at a ratio of 1:1:1, add water to prepare a solution with a concentration of 2g / L, stir at a temperature of 10°C to dissolve it; then add shell β-glucosidase 10000IU and rhamnosidase 3000IU with a glycan mass ratio of 1:...

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Abstract

The invention belongs to the field of biomedicines and in particular relates to a method for preparing an immobilized enzyme and hydrolyzing diosgenin. The method comprises the following steps: crushing a dried turmeric raw material; adding water and immersing; carrying out enzymolysis and removing impurities, extracting dioscin and hydrolyzing the dioscin into the diosgenin with an immobilized enzyme preparation composition; extracting aviation kerosene to prepare a finished product. By utilizing an immobilized enzyme technology, rhamnosidase and beta-glucosidase, which have an expensive market price, are successfully applied to an industrial product with a lower additional value. A biological method is used for replacing traditional dioscin acidolysis, so that the environmental pollution is remarkably reduced; the method has moderate reaction conditions and a low cost.

Description

technical field [0001] The invention relates to a preparation of immobilized enzyme and a method for hydrolyzing diosgenin, belonging to the field of biomedicine. [0002] technical background [0003] Diosgenin is a white or slightly yellow crystalline powder, which is called "medicinal gold" by the medical field, and its medicinal value is very important and broad. my country's diosgenin is a hormone raw material with stable growth and shortage in the world. In the history of the world's steroidal pharmaceutical raw materials, my country's diosgenin has always been in a dominant and monopoly position, occupying 97% of the world's steroidal pharmaceutical raw material market share. Although other developed countries have been committed to finding and making substitutes for decades, due to the defects of natural climate and geographical conditions and raw materials, the cost of substitutes is high and the utilization rate of products is low, so that the developed countries of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10C12P33/20C12P33/06
CPCC12N9/2402C12N11/10C12P33/06C12P33/20C12Y302/01021C12Y302/0104C12Y302/01043
Inventor 苏二正陈龙
Owner 华慧海洋多糖生物技术(深圳)有限公司
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