Molecular marker of loin-eye area character-related gene SVEP1 and application of molecular marker

A technology related to molecular markers and molecular markers, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve problems such as stress caused by measuring environmental influences, affecting breeding effects, time-consuming and labor-intensive, etc.
CN105969845AInactive Publication Date: 2016-09-28HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2016-09-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of preparation of a swine molecular marker, in particular to a molecular marker of a loin-eye area character-related gene SVEP1 and an application of the molecular marker. According to the molecular marker, the sequence of the SVEP1 gene comprises an SNP site which is located on the 44660bpth part of a nucleotide sequence shown as SEQ ID NO.1, and the site is an A / G base mutation which causes SfanI-RFLP polymorphism. The base mutation, which is located within the 3rd intron of the SVEP1 gene, cannot change an amino acid sequence which is translated by the base mutation. Therefore, a sequence, which is shown as SEQ ID NO.2, of the molecular marker can be obtained by designing a primer nearby the SNP site of the SVEP1 gene sequence, and subsequently, loin-eye area characters can be identified. According to the patent, on the basis of an existing PCR-RFLP technology, the A / G mutation is discovered on the 44660bpth part of the SVEP1 gene, and it discovers that the polymorphic site is significantly related to the loin-eye area characters; therefore, the patent is helpful for swine growth character researches to a certain degree.
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Description

technical field

[0001] The invention relates to the field of porcine molecular marker preparation, in particular to a molecular marker of eye muscle area trait-related gene SVEP1 and its application. Background technique

[0002] Animal husbandry occupies a very important position in the development of modern agriculture, and its ratio in agricultural production is usually used as an important indicator to measure the development level of a country and region. Pork is the most important source of meat in China, and the pig breeding industry occupies a pivotal position in my country's animal husbandry. In the mid-1980s, my country’s meat output was about 20 million tons, and pork accounted for about 85%. In 2012, pork output reached 53.35 million tons, accounting for about 63.63% of the total meat. Although the proportion of pork has gradually decreased, it has always occupied a dominant position.

[0003] With the development of society and the improvement of consumption l...

Claims

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