Identification and utilization method of a broad-compatibility recessive sterile line in rice

Abstract

The invention belongs to the field of molecular breeding of crops, and relates to an identifying and utilizing method of a rice wide-compatibility recessive male nuclear sterile line. Nonglutinous rice 'Teqing' and the wide-compatibility variety '02428' are adopted for constructing a recombinant inbred line (RIL), a selfing line with the fertility feature is selected, a novel sterile line RI127S is cultivated and screened, and the sterile line can be used as an intermediate material for rice rotational breeding. The identifying and utilizing method has the advantages that (1) the sterility of the male sterile line is controlled by recessive genes, and is not influenced by length of illumination and temperature under the normal growth condition, and the male sterile line is an ideal recurrent selection intermediate material for rice; (2) the sterile line RI127S contains wide-compatibility genes, the genome of the sterile line RI127S is a mixture of nonglutinous rice and japonica rice varieties, after hybridization with nonglutinous rice and japonica rice, the hybridization seeds are fertile, the F1 fruitage rate of nonglutinous rice is 85%, and the F1 fruitage rate of japonica rice is 80%; (3) the inbred line RI127 has the high yield property intrinsically, and can be taken as a parent of the good stock, and when the RI127 is taken as the sterile line, the process of artificial emasculation for hybridization can be saved.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to the utilization of a wide-compatibility recessive male sterile line in rice breeding. Background technique [0002] Fertility is an important trait of rice and is of great significance in rice production. The research on male sterility in rice was first conducted in the 1920s. Since the 1960s, many different genetic types of male sterile rice materials have been discovered, most of which are recessive genital sterility, such as photothermosensitive male sterility. In the 1970s and 1980s, the location of the rice male sterility gene was mainly based on the analysis of rice trisomies and trait markers to determine the chromosome where the gene is located, such as the recessive genic male sterile genes ms-1, ms-7, ms- 8. ms-9, ms-10, and ms-14 (Khush G S et al., 1991), now mainly use molecular markers to construct genetic linkage maps to locate genes, such as photosensitive s...

AI Technical Summary

Problems solved by technology

However, due to the semi-sterile characteristics of indica-japonica hybrids, indica-japonica heterosis encounters great difficulties in practical application, including a series of problems such as high plant height, poor flowering period, and low seed-setting rate. The seed setting rate of hybrid F1 generation is low, and the seed setting rate of most hybrids is between 5% and 40%.

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