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Rhizome bletillae tissue culture medium, preparation method and application

A tissue culture medium and a culture medium technology, applied in the field of plant tissue culture, can solve the problem of high cost, achieve the effects of low cost, thick stems and branches, and reduced production cost

Inactive Publication Date: 2016-10-12
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The shortcoming of prior art is, the culture medium that is used to improve white and germination rate is to add many other components on the basis of MS culture medium, and cost is higher

Method used

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  • Rhizome bletillae tissue culture medium, preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The white and tissue culture medium of embodiment 1 contains the following components in every liter of 1 / 2 MS medium: naphthaleneacetic acid 0.5mg, paclobutrazol 0.3mg, sucrose 20g, potato 20g and banana peel 80g, and the pH of the medium is 6.2.

[0034] The above medium was prepared according to the following steps:

[0035] Step 1, prepare 1 / 2MS medium according to the conventional method;

[0036] Step 2, according to the formula ratio containing 0.5mg naphthaleneacetic acid, 0.3mg paclobutrazol, 20g sucrose, 20g potatoes and 80g banana peels in each liter of 1 / 2MS medium, respectively weigh naphthaleneacetic acid, paclobutrazol, sucrose, potatoes and banana peels;

[0037] Step 3, cutting potatoes and banana peels into cubes of 0.5cm×0.5cm×0.5cm to obtain potato pieces and banana peel pieces;

[0038] Step 4, mix the naphthaleneacetic acid, paclobutrazol, sucrose weighed in step 2, and the potato pieces and banana peel pieces in step 3, and add the prepared 1 / 2MS...

Embodiment 2

[0041] The white and tissue culture medium of embodiment 2 contains the following components in every liter of 1 / 2 MS medium: naphthaleneacetic acid 0.4mg, paclobutrazol 0.2mg, sucrose 25g, potato 30g and banana peel 100g, and the pH of the medium is 6.0.

[0042] The above medium was prepared according to the following steps:

[0043] Step 1, prepare 1 / 2MS medium according to the conventional method;

[0044] Step 2, according to the formula ratio containing 0.4mg naphthaleneacetic acid, 0.2mg paclobutrazol, 25g sucrose, 30g potatoes and 100g banana peels in each liter of 1 / 2MS medium, respectively weigh naphthaleneacetic acid, paclobutrazol, sucrose, potatoes and banana peels;

[0045] Step 3, cutting potatoes and banana peels into cubes of 0.5cm×0.5cm×0.5cm to obtain potato pieces and banana peel pieces;

[0046] Step 4, mix the naphthaleneacetic acid, paclobutrazol, sucrose weighed in step 2, and the potato pieces and banana peel pieces in step 3, and add the prepared 1 / 2...

Embodiment 3

[0049] The white and tissue culture medium of embodiment 3 contains the following components in every liter of 1 / 2 MS medium: naphthaleneacetic acid 0.6mg, paclobutrazol 0.5mg, sucrose 22g, potato 40g and banana peel 95g, and the pH of the medium is 5.8.

[0050] The above medium was prepared according to the following steps:

[0051] Step 1, prepare 1 / 2MS medium according to the conventional method;

[0052]Step 2, according to the formula ratio containing 0.6mg naphthaleneacetic acid, 0.5mg paclobutrazol, 22g sucrose, 40g potatoes and 95g banana peels in each liter of 1 / 2MS medium, respectively weigh naphthaleneacetic acid, paclobutrazol, sucrose, potatoes and banana peels;

[0053] Step 3, cutting potatoes and banana peels into cubes of 0.5cm×0.5cm×0.5cm to obtain potato pieces and banana peel pieces;

[0054] Step 4, mix the naphthaleneacetic acid, paclobutrazol, sucrose weighed in step 2, and the potato pieces and banana peel pieces in step 3, and add the prepared 1 / 2MS ...

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Abstract

The invention discloses a rhizome bletillae tissue culture medium. Each liter of 1 / 2MS culture medium comprises 0.4-0.6 mg of naphthalene acetic acid, 0.2-0.5 mg of paclobutrazol, 20-25 g of cane sugar, 20-40 g of potatoes and 80-120 g of banana peel. The pH of the culture medium is 5.8-6.2. The invention further provides a preparation method of the rhizome bletillae tissue culture medium and application of the culture medium in rhizome bletillae protocorm induced culture. Waste banana peel serves as one of preparation materials of the culture medium, and other components are added on the basis, so that it is guaranteed that the germination rate of rhizome bletillae seeds is 95% or above, the protocorm differentiation rate is 98% or above, and manufacturing cost of the culture medium is reduced by at least 60%. Besides, compared with a traditional culture medium, rhizome bletillae tissue cultured through the culture medium is plump in mesophyll, thick and strong in stem and branch and better in growth condition, and the rhizome bletillae survival rate is increased by 50%.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a bletilla tissue culture medium, and also relates to a preparation method and application of a bletilla tissue culture medium. Background technique [0002] Bletilla striata, also known as Bletilla striata, is a perennial herbaceous plant belonging to Orchidaceae Bletilla striata, which belongs to the national second-class endangered plant. Its dried rhizomes are used as medicine. It has the effect of astringent to stop bleeding, reduce swelling and promote muscle growth, and is used to treat diseases such as tuberculosis and hemoptysis, gastric ulcer and traumatic bleeding. On the other hand, Baiji tubers are rich in Baiji gum, Baiji polysaccharides, and are widely used in the food industry and daily chemical industry. [0003] There are 30,000 to 50,000 seeds in the pod of Baiji fruit, and the germination rate is only 5% under natural conditions, whic...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 陈康健王喆之强毅胡喜风
Owner SHAANXI NORMAL UNIV