Three-dimensional metal-enhanced fluorescent/colorimetric dual-mode paper chip and ATP measurement
A metal-enhanced fluorescence, paper chip technology, used in fluorescence/phosphorescence, measurement devices, material analysis by optical means, etc., can solve the problems of limited application, poor fluorescence effect signal, etc. The effect of accurate fluorescence measurements
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Embodiment 1
[0036] Application of three-dimensional metal-enhanced fluorescence / colorimetric dual-mode paper chip in ATP detection:
[0037] (1) Design the hydrophobic wax printing area and hydrophilic working area of the hollow channel paper chip. The diameter of the hydrophilic area of the right paper chip is 6 mm, and the gray area of the left paper chip is completely corresponding to the hydrophilic area of the right paper chip. The 6 mm hole in the hole is connected by a hydrophilic channel with a length of 5 mm and a width of 2 mm;
[0038] (2) Print the filter paper chip designed in step (1) on a hydrophobic pattern with a Fuji Xerox wax spray printer;
[0039] (3) Heat the paper chip obtained in step (2) at 60-150 ºC for 1-2 minutes to melt the wax and form a hydrophobic layer;
[0040] (4) Put the paper chips obtained in step (3) on the laser cutting machine, cut off the gray area to form holes;
[0041](5) Grow three-dimensional gold-silver nanostars on the working are...
Embodiment 2
[0049] The preparation steps are the same as Example 1, except that the paper chip used is chromatographic paper.
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