Imaging device and method for quickly acquiring 3D structure information and molecular phenotype information of large sample

Abstract

The invention discloses an imaging device and a method for quickly acquiring 3D structure information and molecular phenotype information of a large sample. The device comprises a sample storage device, a 3D mobile station used for driving the sample storage device to move in a 3D space, a vibration slicing module used for slicing a sample to get the shallow part of the sample, and a wide-field optical microscopic imaging module used for performing high-throughput tomography imaging on the shallow part of the sample. The defects in the prior art, for example, the sample preparation process is complex, the sample shape and fluorescence signals are affected, and imaging is slow, are solved. The 3D structure information of a sample can be quickly acquired and analyzed, and the acquired data has the characteristic of auto-registration. Moreover, molecular phenotype dyeing is performed on a sample slice of a part of interest to get the molecular phenotype information of the sample, and the molecular phenotype information can be registered into the acquired 3D structure information.

Description

technical field [0001] The invention relates to microscopic imaging, in particular to an imaging device and method for rapidly acquiring three-dimensional structure information and molecular phenotype information of large samples. Background technique [0002] The brain is one of the most complex systems in nature, governing all human activities. The exploration of the brain has always been the goal of human research, but until now we still cannot accurately describe the mechanism of intelligence, thinking and consciousness. Neural structure is an important basis for the realization of advanced brain functions. Complex brain functions require the participation of multiple brain regions and are coordinated by local and long-range neural circuits. Brain diseases are often accompanied by structural abnormalities in functionally related specific neural circuits and their input connections and output projection relationships. In order to decipher the structural basis of brain ...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a device and method for quickly obtaining three-dimensional structure information and molecular phenotype information of large samples. Due to the shortcomings of slow signal and imaging speed, the three-dimensional structure information of the sample can be quickly obtained and analyzed. The obtained data has the characteristics of self-registration, and the molecular phenotype staining is performed on the sample slices of the interested part to obtain the molecular phenotype information of the sample. And can be registered to the acquired 3D structural information

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