Compound for inhibiting germination and growth of weed seeds
A technology of weed seeds and compounds, which is applied in the field of research on herbicidal active substances of botanical origin, and achieves the effect of broad application and development prospects
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Embodiment 1
[0022] The significant effect of brucetin D on the seed germination and growth of the monocotyledonous wild oat (Avena fatua L.) was screened from its pure product in different concentration aqueous solutions.
[0023] 1. Preparation of Bruceabrucein D pure product solution
[0024] Prepare 7 concentrations of brucein D aqueous solutions, respectively 6.25 μg / mL, 12.5 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL, and clean water was used as the blank control.
[0025] 2. Wild oat seed treatment
[0026] Select wild oat seeds with the same size and full grains, soak them in 2.5% NaClO solution, and stir them with a magnetic stirrer for 10 minutes, then wash them with sterile water for 3 times (3 minutes each time), dry them with absorbent paper, and store them at 25°C. Under the germination for 24 hours, the wild oat seeds with the same dew and white color were selected for determination.
[0027] Spread double layers of filter paper in a glass Petri dish (diame...
Embodiment 2
[0043] The significant effect of Brucella D on seed germination and growth of spontaneous rape (Brassica napus L.) was screened from its pure product in different concentration aqueous solutions.
[0044] 1. Preparation of Bruceabrucein D pure product solution
[0045] Prepare brucein D aqueous solution with 7 concentrations. They were 6.25 μg / mL, 12.5 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL, and clear water was used as the blank control.
[0046] 2. Rapeseed seed treatment
[0047] Select wild oat seeds with the same size and full grains, soak them in 2.5% NaClO solution, stir them with a magnetic stirrer for 10 minutes, then wash them with sterile water for 3 times (3 minutes each time), blot them dry with absorbent paper, and store them at 25°C. Next germination 24h. Pick wild oat seeds with consistent dew and white color for determination.
[0048] Spread double layers of filter paper in a glass Petri dish (diameter 9 cm) after autoclaving, and add p...
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