Wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use

A wheat seed and persistence technology, applied in the direction of recombinant DNA technology, DNA/RNA fragments, microbial measurement/inspection, etc., can solve the problem of unclear role of seed dormancy persistence

Active Publication Date: 2016-10-26
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these research results are all based on the identification of seed dormancy/ear germination resistance in a short period of time after harvest, and there is no continuous identification of seed dorman

Method used

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  • Wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use
  • Wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use
  • Wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1 material

[0023] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified. The reagents and other materials used are all commercially available products unless otherwise specified. The germplasm resources used can all be obtained from national species The quality resource library is obtained.

[0024] 2 methods

[0025] Eleven varieties of wheat were selected: Annong 8455, Xuke 1, Jimai 20, Suining Tuotuomai, Zhoumai 25, Zi 0706, Wannong 606, Longke 0901, Aifengzao 8, Jing 411, Wanxian Bai For wheat, the identification of seed dormancy persistence ability includes the following steps:

[0026] 2.1 Extraction of wheat genomic DNA by SDS-Tris saturated phenol method

[0027] (1) Take 2-3 wheat seeds and place them in a 2mL sterilized centrifuge tube, and use an electric grinder to grind the seeds into powder;

[0028] (2) Add 1.2mL DNA extraction buffer (200mM Tris-Cl, 250mM NaCl, 25mM EDTA, 0.5% SDS, 2%...

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Abstract

The invention discloses a wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use. The SNP is located at a wheat 2AL chromosome, is tightly linked to a SSR label Xwmc658 in a genetic map and is a novel seed dormancy persistence main-effect site. The CAPS label developed on this basis provides a novel method for wheat preharvest sprouting resistance molecular marker-assistant breeding, lays the foundation for cultivation of a wheat specie with good preharvest sprouting resistance persistence, fills in the blank of continuous seed dormancy/preharvest sprouting resistance identification and is conducive to wheat preharvest sprouting resistance polygene polymerization breeding.

Description

technical field [0001] The invention relates to the technical field of wheat molecular breeding, in particular to a SNP marker significantly related to wheat seed dormancy persistence, a CAPS marker and application thereof. Background technique [0002] Pre-harvest sprouting (PHS) is prone to occur when wheat encounters continuous rainy weather before harvest. Spike germination leads to the consumption of internal storage materials of seeds, resulting in reduced yield and deterioration of quality. If the rainy or high-humidity weather before harvest lasts for a long time, the damage to ear germination will be more serious. Therefore, cultivating wheat varieties with strong ear germination resistance and persistence can help reduce the risk of ear germination caused by prolonged rainy and high humidity weather. Seed dormancy is considered to be the main genetic factor for ear germination resistance. The longer the duration of seed dormancy, the stronger the persistence of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 朱玉磊王升星张海萍常成吴曾云曹佳佳姜昊刘凯秦朦卢杰司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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