Quick sensibilization detecting method of organophosphorus pesticide
An organophosphorus pesticide and detection method technology, applied in the field of chemical analysis, can solve the problems of food safety risks, false negative samples, low detection amount, etc., and achieve the effects of convenient rapid detection, simple operation and high sensitivity
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Embodiment 1
[0027] A rapid sensitization detection method for organophosphorus pesticides:
[0028] For sample extraction, weigh 10 g of homogenized and crushed mixed organophosphorus pesticide samples and mix them with 25 mL of acetone, vortex and oscillate for 10 min, then filter to remove impurities, dry the acetone with 35 ° C nitrogen, add phosphate buffer to set Capacity up to 1000uL;
[0029] For sensitization treatment, add 300uL of 0.05% calcium hypochlorite solution to 1000uL sample extract, oxidize at 30°C for 15min, then add 300uL of 10% sodium nitrite solution, and reduce at 30°C for 15min to obtain the test solution;
[0030] For enzyme inhibition detection, take 100uL of distilled water and 75uL of the test solution in the microtiter plate, then add 50uL of the working enzyme solution acetylcholinesterase, mix well, and inhibit at a constant temperature of 35°C for 15min, then add 50uL of the reaction substrate ATChI and 25uL of chromogen 5,5-dithio-2,2-dinitrobenzoic acid...
Embodiment 2
[0032] A rapid sensitization detection method for organophosphorus pesticides:
[0033] For sample extraction, weigh 5 g of homogenized and crushed mixed organophosphorus pesticide samples and mix them with 20 mL of acetone, vortex and oscillate for 5 min, then filter to remove impurities, dry the acetone with nitrogen at 35 ° C, add phosphate buffer to set Capacity up to 1000uL;
[0034] For sensitization treatment, add 50uL of 0.05% calcium hypochlorite solution to 1000uL sample extract solution, oxidize at 30°C for 10min, then add 50uL of 10% sodium nitrite solution, and perform reduction reaction at 30°C for 10min to obtain the test solution;
[0035] For enzyme inhibition detection, take 50uL of distilled water and 50uL of the test solution in the microtiter plate, then add 30uL of the working enzyme solution acetylcholinesterase, mix well, and inhibit at a constant temperature of 35°C for 3min, then add 30uL of the reaction substrate ATChI and 15uL of chromogen 5,5-dith...
Embodiment 3
[0037] A rapid sensitization detection method for organophosphorus pesticides:
[0038] For sample extraction, weigh 20 g of homogenized and crushed mixed organophosphorus pesticide samples and mix them with 30 mL of acetone, vortex and oscillate for 30 min, then filter to remove impurities, dry the acetone with nitrogen at 35 ° C, add phosphate buffer to set Capacity up to 1000uL;
[0039] For sensitization treatment, add 500uL of 0.05% calcium hypochlorite solution to 1000uL sample extract solution, oxidize at 30°C for 40 minutes, then add 500uL of 10% sodium nitrite solution, and perform reduction reaction at 30°C for 40 minutes to obtain the test solution;
[0040]For enzyme inhibition detection, take 200uL of distilled water and 100uL of the test solution in the microtiter plate, then add 80uL of the working enzyme solution acetylcholinesterase, mix well, and inhibit at a constant temperature of 35°C for 30min, then add 80uL of the reaction substrate ATChI and 40uL of ch...
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