Method for increasing seedling forming rate of grape seeds

Abstract

The invention provides a method for increasing the seedling forming rate of grape seeds. The method comprises the steps that 1, fruits are harvested 5-7 days delayed after being fully ripe, the diseased fruits are removed, the fruits are stored for 1 month to 3 months at the temperature of 0 DEG C-1 DEG C in bags; 2, the seeds are separated, washed clean and stored in sand immediately; 3, the seeds are taken out, washed clean, soaked in 1.5% glacial acetic acid for 24 h, washed with sterile water 3-5 times, poured into mixed liquid of IBA with the concentration of 200 mg/L and LNAA with the concentration of 500 mg/L to be soaked for 24 h and then placed in an incubator at the temperature of 24+/-2 DEG C for dark culturing and root promoting; 4, when the mouths of the grape seeds are cracked and white root tips come out, plug seeding is conducted in a greenhouse; 5, after the seeds are germinated, water is sprayed once to twice every day, the seeds are covered with plastic film for heat preservation at night, and the plastic film is removed after 10 a.m. in the daytime, so that the condition that seedlings are damaged by too high temperature is prevented; 6, when 3-4 true leaves of the seedlings grow out, the seedlings are transplanted to nutrient soil in non-woven fabric bags, water is sprayed to the leaf surfaces 1-2 times every day in the first weak after transplanting, and shading is conducted. According to the method, the seedling forming rate of the grape seeds is significantly increased.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for improving the seedling rate of grape seeds. Background technique [0002] Hybrid breeding and seed selection play an important role in the breeding of new grape varieties. At present, hybrid breeding is still one of the main methods of grape breeding, and hybrid seedlings are cultivated from hybrid or self-bred seeds. It is an important link. The number of hybrid seedlings obtained directly affects the efficiency of hybrid breeding. Normally, the germination rate of grape seeds is low, generally 30% to 50%, and the final seedling rate will be even lower. The main reasons are: insufficient development of embryos in grape seeds, pseudoparthenogenesis in some varieties, hard seed coat, etc. The low seedling rate after germination is mainly caused by improper management. Therefore, it is of great significance to study and improve the germination rate and ...

AI Technical Summary

Problems solved by technology

The main reasons are: insufficient development of embryos in grape seeds, pseudoparthenogenesis in some var