A dynamic culture instrument for tissue engineering

Abstract

The invention relates to a dynamic cultivation instrument for tissue engineering. Different from the prior art, the dynamic cultivation instrument comprises a box, a driving motor, a rotating support base, an attracting block, a cultivation bottle and a rotor. The driving motor is arranged in the box, and the power output end of the driving motor is vertically upward. The rotating support base is fixedly connected with the power output end of the driving motor. The attracting block is arranged on the rotating support base. The cultivation bottle is arranged on the upper surface of the box, and the bottom of the cultivation bottle faces the driving motor directly. The rotor is disposed in the cultivation bottle. At least one of the rotor and the attracting block is made of magnetic materials, and the rotor and the attracting block attract each other. The cultivation instrument is simple in structure, low in cost, safe, reliable and high in applicability.

Description

technical field [0001] The invention relates to the field of biomedical tissue engineering, in particular to a dynamic culture instrument for tissue engineering. Background technique [0002] Tissue engineering is an emerging discipline that combines cell biology and material science to construct tissues or organs in vitro or in vivo. Its basic principle is to obtain a small amount of living tissue from the body, use special enzymes or other methods to separate cells (also known as seed cells) from the tissue for culture and expansion in vitro, and then combine the expanded cells with good biological phase. Capacitive, degradable, and absorbable biomaterials (scaffolds) are mixed in a certain proportion to allow cells to adhere to the biomaterials (scaffolds) to form a cell-material complex; a tissue or organ disease in which the complex is implanted into the body As the biomaterials are gradually degraded and absorbed in the body, the implanted cells continue to proliferat...

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Problems solved by technology

In dynamic culture, it is usually necessary to use a dynamic incubator with stirring function and place it in an incubator under a sterile environment for cultivation. However, the current dynamic incubator is expensive, and it is difficult for laboratories in domestic universities , can not be purchased in batches; second, the volume is large, a standard incubator (inner diameter 46x46x48cm) can accommodate up to 4 instruments for a single seat, and 1 instrument for four seats. To give different mechanical stimuli to different tissues, multiple instruments are required. One incubator is difficult to implement. When using four-seat culture instruments, the stirring speed of the four seats is the same, which cannot meet the needs of experimental grouping; third, the base instrument cannot be sterilized by high temperature and high pressure, and it is easy to damage the culture environment; fourth, use 220V AC Power supply, after being placed in the incubator as a whole, the risk is high, the diameter of the wire is large, and it can only enter through the door crack of the incubator, destroying the closed and stable cultivation environment in the incubator, and accelerating the aging of the incubator; the fifth is the stirring of the culture solution The method is generally realized by the rotation of the stirring rod or the stirring blade inserted into the culture bottle, because the volume of the culture bottle is generally not large, and inserting the stirring rod or stirring blade will make the space in the culture bottle more cramped, so that it cannot accommodate larger engineering organization

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