Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for increasing yield of F VII in human prothrombin compound

A technology for human prothrombin and complexes, which is applied in the field of extracting human prothrombin complexes, can solve the problems that need to be improved, and achieve the effect of high yield, high purity and high yield

Inactive Publication Date: 2016-11-09
WUHAN ZHONGYUAN RUIDE BIOLOGICAL PROD CO LTD
View PDF9 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, current methods for obtaining human prothrombin complexes still need to be improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for increasing yield of F VII in human prothrombin compound

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0052] Preparation of S / D stock solution (take 1L as an example): Dissolve 110ml Tween-80 in 857ml water for injection, after fully dissolved, add 33.3ml TNBP, continue to stir until clear to obtain 1L S / D solution, cool down to 30°C The following spare.

[0053] Plasma: from Enshi Tianyuan Plasmapheresis Station Co., Ltd., batch numbers: 20150914, 20151015, 20151120.

[0054] Detection method:

[0055] A. Conductivity detection is realized by a conductivity meter, which is purchased from Mettler, model FE30Kplus, and its detection range is 0.00-199.9ms / cm.

[0056] B. Potency detection: coagulation method is used. Instrument: French STAGO company automatic coagulation analyzer, model: STA CompactMax. specific method:

[0057] 1) Take 1ml of water for injection to dissolve the standard substance, add physiological saline to dilute it to 1IU / ml, and finally dilute the standard substance to 1 / 2, 1 / 4, 1 / 6, 1 / 8 with diluent. Load factor deficient plasma, coagulation quality con...

Embodiment 1

[0061] In this example, the human prothrombin complex was extracted as follows:

[0062] (1) Provide frozen plasma, alcohol-sterilized plasma bags, and inject hydrated plasma.

[0063] (2) Separate the plasma with a tubular centrifuge at 0-4°C, collect 500 L of supernatant (ie cryoprecipitated plasma), and adjust its pH to 7.3.

[0064] (3) The cryoprecipitate-free plasma is heated to 15-20°C.

[0065] (4) According to the ratio of 1.5kg dry glue / 1000L cryoprecipitated plasma, add the wet glue which has been pre-balanced with the balance solution to the cryoprecipitated plasma, and stir and absorb at 17°C for 40 minutes. The plasma and gel are transferred to the chromatographic column, the gel is collected, and the effluent is used for the production of albumin and static acrylic acid. The concentration of sodium chloride in the equilibrium solution is 0.10mol / L, the concentration of sodium citrate is 0.015mol / L, pH: 7.2.

[0066] (5) Wash the gel 3 times with the washing s...

Embodiment 2

[0075] In this example, the human prothrombin complex was extracted as follows:

[0076] (1) Provide frozen plasma, alcohol-sterilized plasma bags, and inject hydrated plasma.

[0077] (2) Separate the plasma with a tubular centrifuge at 0-4°C, collect 500 L of supernatant (ie cryoprecipitated plasma), and adjust its pH to 7.3.

[0078] (3) The cryoprecipitate-free plasma is heated to 15-20°C.

[0079] (4) According to the ratio of 1.5kg dry glue / 1000L cryoprecipitated plasma, add the wet glue which has been pre-balanced with the balance solution to the cryoprecipitated plasma, and stir and absorb at 17°C for 40 minutes. The plasma and gel are transferred to the chromatographic column, the gel is collected, and the effluent is used for the production of albumin and static acrylic acid. The concentration of sodium chloride in the equilibrium solution is 0.12mol / L, the concentration of sodium citrate is 0.015mol / L, pH: 7.2.

[0080] (5) Wash the gel 3 times with the washing s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting equilibrium liquid in a human prothrombin compound and extracting the human prothrombin compound. The equilibrium liquid for extracting the human prothrombin compound contains 0.10mol / L to 0.15mol / L of sodium chloride and 0.01mol / L to 0.02mol / L of sodium citrate, and the pH value of the equilibrium liquid is 6.8 to 7.2. By utilizing the equilibrium liquid, the human prothrombin compound can be effectively extracted, the yield and purity are relatively high, and particularly the yield of a blood coagulation factor VII (F VII) is relatively high. In addition, the stability of the obtained human prothrombin compound is relatively good.

Description

technical field [0001] The present invention relates to the field of biology. Specifically, the present invention relates to a method for extracting human prothrombin complex. Background technique [0002] Human prothrombin complex concentrate (PCC) mainly includes coagulation factor II (Factor II, FII), coagulation factor VII (Factor VII, FVII), coagulation factor IX (Factor IX, FIX), coagulation factor X (Factor X, FX) four coagulation factors, which are traditionally mainly used in the treatment of hemophilia B. With the approval of clinical application, the role of PCC in hemostasis has been paid more and more attention by clinicians, and its hemostatic effect largely depends on FⅦ. The higher the FVII content in PCC, the better the hemostatic effect. [0003] CN 101439047A discloses a process for improving the yield of human prothrombin complex stability FVII, including: fresh low-temperature frozen healthy human plasma→clarification and filtration→adjustment of ioni...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/64C07K14/75
CPCC12N9/6437C07K14/75C12N9/6432C12N9/644C12Y304/21006C12Y304/21021C12Y304/21022
Inventor 辛叶梁华肖雷王玉兵徐建生
Owner WUHAN ZHONGYUAN RUIDE BIOLOGICAL PROD CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com