A kind of polypeptide modified honeycomb cellulose support and preparation method thereof
A honeycomb, cellulose technology, applied in biochemical equipment and methods, cell culture supports/coatings, prostheses, etc., can solve problems such as unsatisfactory biodegradability, and achieve excellent biocompatibility and connectivity. Good and wide source of raw materials
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Embodiment 1
[0029] Preparation of Honeycomb Cellulose Scaffolds Modified by Poly(L-glutamic acid-γ-benzyl ester)
[0030] 1) Processing and forming: the creeper rattan is processed into a disc with a thickness of 4 mm and a diameter of 6 mm to obtain a sample;
[0031] 2) Alkali treatment: soak the sample obtained in step 1) in a sodium hydroxide solution with a concentration of 4 mol / L for 12 hours, then transfer the sample to a 2.5 mol / L aqueous sodium hydroxide solution, heat and stir at 100°C for 10 Hour;
[0032] 3) Acid treatment: the sample after the alkali treatment in step 2) is fully washed with distilled water, then placed in hydrochloric acid with a concentration of 2mol / L, heated and stirred at 100°C for 4 hours;
[0033] 4) Bleaching: place the sample after the acid treatment in step 3) in a sodium hypochlorite solution with a concentration of 2 mol / L, take it out after stirring at room temperature for 6 hours, rinse repeatedly with water and ethanol until the pH is neutral...
Embodiment 2
[0040] 1) Processing and molding: processing the creeper rattan into a disc with a thickness of 1 mm and a diameter of 0.5 cm to obtain a wood sample.
[0041] 2) Alkali treatment: after soaking the wood sample in step 1) in a sodium hydroxide solution with a concentration of 1mol / L for 24 hours, the sample is transferred to an aqueous solution of sodium hydroxide with a mass concentration of 12.5mol / L. Heat and stir at 60°C for 16 hours.
[0042] 3) Acid treatment: wash the sample after step 2) alkali treatment, put it into hydrochloric acid with a concentration of 1 mol / L, heat and stir at 65°C for 12 hours.
[0043] 4) Bleaching: Place the acid-treated sample in step 3) in a sodium hypochlorite solution with a concentration of 1 mol / L, take it out after stirring at room temperature for 4 hours, rinse it repeatedly with water and ethanol until the pH is neutral, and then dry it.
[0044] 5) Polypeptide coating: the sample bleached in step 4) is placed in the polypeptide sol...
Embodiment 3
[0046] 1) Processing and forming: the Paulownia is processed into a disc with a thickness of 10 mm and a diameter of 2 cm to obtain a wood sample.
[0047] 2) Alkali treatment: after soaking the wood sample in step 1) in a sodium hydroxide solution with a concentration of 3mol / L for 18 hours, the sample is transferred to an aqueous solution of sodium hydroxide with a mass concentration of 5mol / L. ℃ and heated and stirred for 6 hours.
[0048] 3) Acid treatment: wash the sample after step 2) alkali treatment, put it into hydrochloric acid with a concentration of 4 mol / L, heat and stir at 70° C. for 10 hours.
[0049] 4) Bleaching: Place the acid-treated sample in step 3) in a sodium hypochlorite solution with a concentration of 1.5 mol / L, take it out after stirring at room temperature for 10 hours, rinse repeatedly with water and ethanol until the pH is neutral, and then dry it.
[0050] 5) Polypeptide coating: the sample bleached in step 4) is placed in the polypeptide soluti...
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