Separating and screening method and applications of chlorpyrifos-degrading bacterium

A chlorpyrifos-degrading bacteria, separation and screening technology, applied in the field of microorganisms and its applications, can solve problems affecting the yield and quality of agricultural products, environmental pollution, and low actual utilization of pesticides

Inactive Publication Date: 2016-11-16
南通市产品质量监督检验所
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the actual utilization rate of pesticides is extremely low, only 1-10% are used on crops, and more than 90% of pesticides either remain on plants and soil or enter water bodies, which not only causes environmental pollution, but also affects the output of agricultural products and quality

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Separating and screening method and applications of chlorpyrifos-degrading bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Screening of chlorpyrifos-degrading bacteria

[0037] Add 5 g of soil to 50 mL of MM medium containing 50 mg / L chlorpyrifos, and culture it on a shaker at 30°C and 120 r / min for 3 days, transfer 2 mL of this mixture into fresh MM medium, Increase the concentration of chlorpyrifos to 100 mg / L, transfer after 3 days and increase the concentration of chlorpyrifos to 200 mg / L, continue to cultivate for 3 days, and then repeat the transfer to MM medium with a concentration of 200 mg / L of chlorpyrifos twice to acclimatize The final bacterial solution was serially diluted 1000 times, and 100 µL was drawn and spread on the NB solid medium with a concentration of 200 mg / L of chlorpyrifos and cultured in a constant temperature incubator at 30°C for 3 days, and a single colony was picked and placed on the NB solid medium with the same concentration , transferred 3 times in a row, and the obtained single colony was named DC1. The colony of DC1 on the NB plate was milky white an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a separating and screening method and applications of chlorpyrifos-degrading bacterium. The separating and screening method and applications of the chlorpyrifos-degrading bacterium are realized through the steps of soil collection, culture medium preparation, chlorpyrifos-degrading bacterium domestication, chlorpyrifos-degrading bacterium separation, bacterial strain identification, bacterial preparation preparation and chlorpyrifos degradation by adopting the bacterial preparation. The inorganic salt culture medium taking chlorpyrifos as the unique carbon source is adopted, the chlorpyrifos-degrading bacterium is domesticated in the field soil in which rice is planted throughout the year and in which the pesticide, namely, chlorpyrifos is frequently used, the degrading bacterium is separated by adopting the solid culture medium so that the single bacterial strain is obtained, the 16SrDNA sequence of the bacterial strain is subjected to sequencing, the species of the bacterial strain is identified by combining with the homology analysis and phylogenetic analysis, and then the bacterial strain is prepared into the bacterial preparation which is used for degrading chlorpyrifos.

Description

technical field [0001] The invention belongs to the field of microorganisms and applications thereof, and in particular relates to a method for separating and screening chlorpyrifos-degrading bacteria and applications thereof. Background technique [0002] Chlorpyrifos, whose scientific name is chlorpyrifos, is a highly efficient, broad-spectrum, and moderately toxic organophosphate insecticide and acaricide, and it is also one of the most produced and sold insecticides in the world. In my country, chlorpyrifos is widely used in agricultural products such as rice, vegetables, melons and fruits, especially after the sale and use of five highly toxic pesticides such as methamidophos and parathion are banned, the use of chlorpyrifos is more extensive and frequent. However, the actual utilization rate of pesticides is extremely low, only 1-10% are used on crops, and more than 90% of pesticides either remain on plants and soil or enter water bodies, which not only causes environm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C12N1/20A62D3/02C12R1/125A62D101/04A62D101/28
CPCC12N1/02A62D3/02A62D2101/04A62D2101/28C12N1/20
Inventor 罗鑫邵彪
Owner 南通市产品质量监督检验所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap