Separating and screening method and applications of chlorpyrifos-degrading bacterium

Abstract

The invention discloses a separating and screening method and applications of chlorpyrifos-degrading bacterium. The separating and screening method and applications of the chlorpyrifos-degrading bacterium are realized through the steps of soil collection, culture medium preparation, chlorpyrifos-degrading bacterium domestication, chlorpyrifos-degrading bacterium separation, bacterial strain identification, bacterial preparation preparation and chlorpyrifos degradation by adopting the bacterial preparation. The inorganic salt culture medium taking chlorpyrifos as the unique carbon source is adopted, the chlorpyrifos-degrading bacterium is domesticated in the field soil in which rice is planted throughout the year and in which the pesticide, namely, chlorpyrifos is frequently used, the degrading bacterium is separated by adopting the solid culture medium so that the single bacterial strain is obtained, the 16SrDNA sequence of the bacterial strain is subjected to sequencing, the species of the bacterial strain is identified by combining with the homology analysis and phylogenetic analysis, and then the bacterial strain is prepared into the bacterial preparation which is used for degrading chlorpyrifos.

Description

technical field [0001] The invention belongs to the field of microorganisms and applications thereof, and in particular relates to a method for separating and screening chlorpyrifos-degrading bacteria and applications thereof. Background technique [0002] Chlorpyrifos, whose scientific name is chlorpyrifos, is a highly efficient, broad-spectrum, and moderately toxic organophosphate insecticide and acaricide, and it is also one of the most produced and sold insecticides in the world. In my country, chlorpyrifos is widely used in agricultural products such as rice, vegetables, melons and fruits, especially after the sale and use of five highly toxic pesticides such as methamidophos and parathion are banned, the use of chlorpyrifos is more extensive and frequent. However, the actual utilization rate of pesticides is extremely low, only 1-10% are used on crops, and more than 90% of pesticides either remain on plants and soil or enter water bodies, which not only causes environm...

AI Technical Summary

Problems solved by technology

However, the actual utilization rate of pesticides is extremely low, only 1-10% are used on crops, and more than 90% of pesticides either remain on plants and soil or enter water bodies, which not only causes environmental pollution, but also affects the output of agricultural products and quality

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