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A kind of marine streptomyces and its application in preparing nosiheptide
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A marine streptomyces and nosiheptide technology, applied in the field of microbiology, can solve the problems of the decline in the number of new antibacterial antibiotics and the inability to meet the needs of infectious disease prevention and treatment, and achieve the effect of broad application prospects
Active Publication Date: 2019-08-13
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology
In stark contrast to this, the number of discoveries of new antibacterial antibiotics has dropped sharply. In the past 10 years, only a few new antibiotics such as daptomycin and tigecycline have been on the market, which is far from meeting the needs of human beings for the prevention and treatment of infectious diseases.
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Embodiment 1
[0023] Embodiment 1: Isolation and identification of marine streptomyces (Streptomyces sp.) SCSIO 1682
[0024] The marine streptomyces (Streptomyces sp.) SCSIO 1682 of the present invention is isolated from a sediment sample at a water depth of 45m in the Luhuitou sea area of Sanya, my country (109°48'E, 18°23'N).
[0025] The taxonomic characteristics of this strain are:
[0026] 1. Morphological characteristics:
[0027] The colony is dry, and the single colony is generally round, small, compact, difficult to spread, and raised in the middle. The hyphae in the base and the aerial hyphae are tightly combined and difficult to stir up. After the final spore production, the spores are easy to scrape. Light brown aerial hyphae and yellow stroma hyphae formed on ISP4 medium.
[0029] Extract the genomic DNA of the above-mentioned isolated bacterial strain, amplify its 16S rDNA sequence by conventional method PCR, and ...
Embodiment 2
[0031] Embodiment 2: Separation and identification of Nosiheptide
[0032] 1. Prepare the fermentation culture of Streptomyces sp. SCSIO 1682:
[0033] (1) Preparation of seed medium and fermentation medium:
[0034] a) Preparation of seed medium: every liter of seed medium contains 10g soybean powder, 5g starch, 2g bacteriological peptone, 20g glucose, 2g yeast extract, 0.5g K 2 HPO 4 , 0.5g MgSO 4 ·7H 2 O, 2g CaCO 3 , 30g sea salt, and tap water as the balance, mix the above components evenly, and adjust the pH to 7.0. Prepare 1L of seed medium, and then evenly distribute it in 20 250mL Erlenmeyer flasks, each containing 50mL of seed medium, and sterilize at 121°C for 30min for later use.
[0035] b) Preparation of fermentation medium: every liter of fermentation medium contains 10g soybean powder, 5g starch, 2g bacteriological peptone, 20g glucose, 2g yeast extract, 0.5g K 2 HPO 4 , 0.5g MgSO 4 ·7H 2 O, 2g CaCO 3 , 30g sea salt, and tap water as the balance, mix ...
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Abstract
The invention discloses a marine streptomyces and its application in preparing nosiheptide. Streptomyces sp. SCSIO 1682 was deposited in the China Center for Type Culture Collection (CCTCC) on June 6, 2016, address: Wuhan University, Wuhan, China, and the deposit number is: CCTCC NO: M 2016307. The fermentation culture of Streptomyces sp. SCSIO 1682 of the present invention can produce nosiheptide, as shown in formula (I). The invention provides a biological preparation method for the production and preparation of nosiheptide, and has broad application prospects.
Description
[0001] Technical field: [0002] The invention belongs to the technical field of microorganisms, and in particular relates to a marine streptomyces (Streptomyces sp.) SCSIO 1682 and its application in preparing nosiheptide. [0003] Background technique: [0004] In recent years, the uncontrolled addition of antibiotics in the field of animal breeding and the long-term, repeated, and high-dose abuse of antibiotics in the clinical field have exacerbated the rapid emergence and spread of various types of drug-resistant pathogens, such as methicillin-resistant Staphylococcus aureus (methicillin-resistant Staphylococcus aureus, MRSA), vancomycin-resistant Enterococcus (VRE), multi-drug-resistant tuberculosis (multidrug-resistant tuberculosis, MDRMT). "NDM-1 producing resistant bacteria (New Delhi-metallo-β-lactamase, New Delhi metallo-β-lactamase)" of "Superbugs". In stark contrast to this, the number of new antibacterial antibiotics has dropped sharply. In the past 10 years, only...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/02C12R1/465
CPCC12P21/02C12N1/205C12R2001/465
Inventor 鞠建华桂春李青连程伟鸽
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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