Preparation method and application of photoelectric aptamer sensor based on ppy/cds/g‑c3n4
An aptamer sensor, g-c3n4 technology, applied in instruments, scientific instruments, material analysis through electromagnetic means, etc., to achieve high-sensitivity detection, simple operation, and wide signal response range
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Embodiment 1
[0027] Example 1 A based on PPy / CdS / g-C 3 N 4 Preparation method of photoelectric aptasensor
[0028] (1) Pretreatment of the ITO electrode: cut the ITO conductive glass to a size of 1.0 cm × 2.5 cm, clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen;
[0029] (2) Add 6 µL, 3 mg / mL polypyrrole sensitized cadmium sulfide / carbon nitride PPy / CdS / g-C dropwise 3 N 4 The solution is placed on the surface of the electrode and dried at room temperature;
[0030] (3) Add 6 μL, 1 μmol / L aptamer solution dropwise to the surface of the electrode, dry it in a refrigerator at 4°C, and rinse with Tris-HCl buffer;
[0031] (4) Drop 6 µL, 1 mmol / L 6-mercaptohexanol solution on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, and dry it in a refrigerator at 4°C;
[0032] (5) Rinse with Tris-HCl buffer solution, let it dry to a wet state, add 6 μL of adenosine solutions with different concentr...
Embodiment 2
[0033] Example 2 A PPy / CdS / g-C based 3 N 4 Preparation method of photoelectric aptasensor
[0034] (1) Pretreatment of the ITO electrode: cut the ITO conductive glass to a size of 1.0 cm × 2.5 cm, clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen;
[0035] (2) Add 6 µL, 4 mg / mL polypyrrole sensitized cadmium sulfide / carbon nitride PPy / CdS / g-C dropwise 3 N 4 The solution is placed on the surface of the electrode and dried at room temperature;
[0036] (3) Add 6 μL, 0.5 μmol / L aptamer solution dropwise to the surface of the electrode, dry it in a refrigerator at 4°C, and rinse with Tris-HCl buffer;
[0037] (4) Add 6 µL, 2 mmol / L 6-mercaptohexanol solution dropwise on the electrode surface to seal the non-specific active sites on the electrode surface, and dry it in a refrigerator at 4°C;
[0038] (5) Rinse with Tris-HCl buffer solution, let it dry to a wet state, add 6 μL of adenosine solutions with different concentrations ...
Embodiment 3
[0039] Example 3 A PPy / CdS / g-C based 3 N 4 Preparation method of photoelectric aptasensor
[0040] (1) Pretreatment of the ITO electrode: cut the ITO conductive glass to a size of 1.0 cm × 2.5 cm, clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen;
[0041] (2) Add dropwise 6 µL, 5 mg / mL polypyrrole-sensitized cadmium sulfide / carbon nitride PPy / CdS / g-C 3 N 4 The solution is placed on the surface of the electrode and dried at room temperature;
[0042] (3) Add 6 μL, 1.5 μmol / L aptamer solution dropwise on the surface of the electrode, dry it in a refrigerator at 4°C, and rinse with Tris-HCl buffer;
[0043] (4) Add 6 µL, 3 mmol / L 6-mercaptohexanol solution dropwise on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, and dry it in a refrigerator at 4°C;
[0044] (5) Rinse with Tris-HCl buffer solution, let it dry to a wet state, add 6 μL of adenosine solutions with different c...
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