Novel cryopreservation agent for rat sperm vitrified cryopreservation
A cryoprotectant and cryoprotectant technology, applied in the field of bioengineering, can solve the problems of low recovery rate of rat sperm cryoprotectant, and achieve the effect of improving vitality and fertilization rate
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[0017] The present invention will be further described below in conjunction with specific embodiments. It should be understood that the following examples are only used to illustrate the present invention but not to limit the scope of the present invention.
[0018] Implementation Example 1: Preparation of soybean milk (bean milk for rat sperm; RS-BM)
[0019] (1) Select 50g of high-quality dry soybeans, wash the floating dust on the surface of soybeans with water (×3 times); put them into a 2000ml glass beaker, add an appropriate amount of double distilled water, and soak overnight (12h);
[0020] (2) Discard the soaking solution and add 1 000ml of purified water for cell culture (culture water);
[0021] (3) Prepare soybean milk liquid. Backgammon Soymilk Machine (A505), program: "Five Grain Soymilk" setting, 30min; cool to room temperature; coarse filter;
[0022] (4) centrifugal. 10 000rpm (or 11 500g), 30min; gently absorb the milky white denatured protein suspension ...
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