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Novel cryopreservation agent for rat sperm vitrified cryopreservation

A cryoprotectant and cryoprotectant technology, applied in the field of bioengineering, can solve the problems of low recovery rate of rat sperm cryoprotectant, and achieve the effect of improving vitality and fertilization rate

Inactive Publication Date: 2016-11-23
INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The primary purpose of the present invention is to solve the problem of low recovery rate of rat sperm cryoprotectant in existing reports

Method used

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Examples

Experimental program
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Embodiment Construction

[0017] The present invention will be further described below in conjunction with specific embodiments. It should be understood that the following examples are only used to illustrate the present invention but not to limit the scope of the present invention.

[0018] Implementation Example 1: Preparation of soybean milk (bean milk for rat sperm; RS-BM)

[0019] (1) Select 50g of high-quality dry soybeans, wash the floating dust on the surface of soybeans with water (×3 times); put them into a 2000ml glass beaker, add an appropriate amount of double distilled water, and soak overnight (12h);

[0020] (2) Discard the soaking solution and add 1 000ml of purified water for cell culture (culture water);

[0021] (3) Prepare soybean milk liquid. Backgammon Soymilk Machine (A505), program: "Five Grain Soymilk" setting, 30min; cool to room temperature; coarse filter;

[0022] (4) centrifugal. 10 000rpm (or 11 500g), 30min; gently absorb the milky white denatured protein suspension ...

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Abstract

The invention discloses a rat sperm cryopreservation agent (RS-CPA) for a SD rat and a gene engineering rat under a SD rat genetic background. The RS-CPA comprises 5% of beam milk extract as a RS-CPA main component and also comprises raffinose. Through use of the beam milk extract, the RS-CPA greatly improves resurgent sperm vitality and a fertilization rate and realizes a refrigeration recovery rate of 10% or more.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular, it relates to an ultra-low temperature refrigerant for rat sperm, and more specifically, it relates to a glass used for SD rats and genetically engineered rats with SD rats as the genetic background. Sperm cryoprotectant. Background technique [0002] The cryopreservation of sperm has a positive effect on animal experiments and can greatly save storage space. One of the key factors affecting the cryopreservation effect is the cryoprotectant used in the cryopreservation process. [0003] Rat (rat) sperm has a relatively low recovery rate after cryopreservation because of its physiological structure different from that of mouse (mouse) and other mammals. Scientists from various countries have been looking for cryoprotectants that are more suitable for rat sperm. [0004] Here we are applying for an original rat sperm vitrification cryoprotective solution and its freezing method. A n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 张连峰刘牧
Owner INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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