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Soybean glycopeptides with bioactivity functions and preparation methods thereof

A bioactive, glycopeptide technology, applied in the field of soybean glycopeptide and its preparation, achieves the effects of high safety, strong specificity and abundant sources

Inactive Publication Date: 2016-11-23
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the preparation of glycoproteins using this enzyme is limited to a few research groups

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of glycopeptides with antioxidant activity:

[0041] The first:

[0042]Disperse the glycosylated soybean protein precipitate in water to obtain a protein dispersion, adjust the pH of the dispersion to 8.0, and the solid-to-liquid ratio is 0.4:10; the amount of solid in the solid-to-liquid ratio is the weight of soybean protein, in kilograms, and the amount of liquid The unit is liter; after stirring evenly, add Alcalase alkaline protease, add 1g of Alcalase alkaline protease per kilogram of protein, stir well, shake at 60°C for 2 hours; ,cool down. Adjust the pH of the solution to 7.0.

[0043] Concentration: Use an evaporator to concentrate the above filtrate to a solid content of 25-55%. Spray drying: The inlet temperature is 110°C, the feed flow rate is 15%, and the air extraction rate is 80%. Under these conditions, the recovery rate of soybean peptide is ≧50%.

[0044] The second type:

[0045] Disperse the precipitate in water to obtain a protein...

Embodiment 2

[0050] Preparation of glycopeptides with antibacterial activity:

[0051] The first:

[0052] Disperse the glycosylated soybean protein precipitate in water to obtain a protein dispersion, adjust the pH of the dispersion to 2.0, and the solid-to-liquid ratio is 0.4:10; the amount of solid in the solid-to-liquid ratio is the weight of soybean protein, in kilograms, and the amount of liquid The unit is liter; after stirring evenly, add pepsin, 2g pepsin per kilogram of protein, stir well, shake at 37°C for 2 hours; after the soybean protein enzymatic hydrolysis reaction, inactivate the enzyme at 85°C for 5 minutes, and cool down. Adjust the pH of the solution to 7.0.

[0053] Concentration: Use an evaporator to concentrate the above filtrate to a solid content of 25-55%. Spray drying: The inlet temperature is 110°C, the feed flow rate is 15%, and the air extraction rate is 80%. Under these conditions, the recovery rate of soybean peptide is ≧50%.

[0054] The second type:

...

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PUM

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Abstract

The invention discloses soybean glycopeptides with bioactivity functions and preparation methods thereof, and particularly relates to soybean glycopeptides with antioxidant activity and soybean glycopeptides with bacteriostatic activity, and preparation methods thereof. According to the preparation method of the soybean glycopeptides with antioxidant activity, firstly, glycosylated soy protein is prepared; the glycosylated soy protein is respectively prepared into soybean glycopeptides with antioxidant activity and soybean glycopeptides with bacteriostatic activity; glycosylated soy protein precipitates are dispersed in water to obtain protein dispersion liquid; Alcalase alkaline protease is added; after uniform stirring, oscillation is performed for 2 hours; after the soy protein enzymolysis reaction is completed, enzyme deactivation is performed for 5min at 85 DEG C; cooling is performed. According to the preparation method of the soybean glycopeptides with bacteriostatic activity, glycosylated soy protein precipitates are dispersed into water to obtain a protein dispersing solution; pepsin is added; the mixture is uniformly stirred; after the soyabean protein enzymatic hydrolysis reaction is completed, the pH of the solution is regulated to 7.0. In the concentration process, the filter liquid is concentrated by an evaporator until the solid content is 25 percent to 55 percent. In the spray drying process, the inlet temperature is 110 DEG C; the feeding flow speed is 15 percent; the air extraction quantity is 80 percent.

Description

technical field [0001] The invention relates to a soybean glycopeptide with biological activity and a preparation method thereof, in particular to a soybean glycopeptide with antioxidant activity and antibacterial activity and a preparation method thereof. Background technique [0002] Soybean protein is a high-quality protein resource with a wide range of sources and low price. It is a traditional food in Asia, especially playing an important role in ensuring the supply of dietary protein in my country. The glycosylation modification of protein is to connect hydrophilic carbohydrates to protein molecules in a covalent manner, so that it has both the macromolecular properties of proteins and the hydrophilic properties of carbohydrates. Glycosylation in the Maillard reaction pathway can improve the functional properties of proteins, but there are defects such as long reaction cycle, product browning, nutrient loss and safety hazards. [0003] The use of transglutaminase can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 宋春丽任健陈佳鹏
Owner QIQIHAR UNIVERSITY