ALL prognostic related gene mutation detection method, primers and kit

Abstract

The embodiment of the invention provides an ALL prognostic related gene mutation detection method, primers and a kit. PCR and sequencing technologies are used, specific primers are mainly used for amplifying specific exon sections of CREBBP genes in genome DNA, sequencing is conducted on target fragments obtained through amplification through a sequencing reaction, and whether mutation exists or not is detected by comparing the obtained sequence with a standard genetic sequence. The detection process is rapid and sensitive, operation is easy, the accuracy is high, and the detection result is definite and clear; the detection specificity is high, a gene mutation detection method which is the most accurate at present is adopted, and purposiveness is high; the blood collection amount of a detection specimen is low, a detected object himself / herself is benefited, fewer experimental instruments are used in the detection process, and the cost is reduced. The invention aims at obtaining analytical data capable of being used for medication guidance and prognosis evaluation of acute lymphoblastic leukemia, and the analytical data can play an important role in the field of medical detection.

Description

technical field [0001] The invention belongs to the technical field of medicine and molecular biology, and in particular relates to a method for detecting mutations of genes related to prognosis of ALL (Acute Lymphoblastic Leukemia, acute lymphoblastic leukemia). The invention also provides primers used in the detection method and a kit containing the primers. Background technique [0002] ALL is a neoplastic disease originating from B-lineage or T-lineage lymphoid progenitor cells. The blast cells proliferate and aggregate abnormally in the bone marrow and inhibit normal hematopoiesis, resulting in anemia, thrombocytopenia and neutropenia; blast cells can also invade Extramedullary tissues, such as meninges, gonads, thymus, liver, spleen, or lymph nodes, cause corresponding lesions. In 2008, the WHO diagnostic criteria for ALL were: cell morphology, immunophenotype, bone marrow primitive and immature lymphocytes ≥ 20%, classified as: ① precursor B-cell acute lymphoblastic ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of insufficient accuracy and convenience in the current ALL prognosis assessment detection technology, the present invention provides a new ALL prognosis-related gene mutation detection method and corresponding primers and kits, which comprehensively apply PCR amplification and gene sequencing technology, which can accurately and quickly detect the mutation of the CREBBP gene related to the prognosis of ALL, and provide an analysis basis for the evaluation of the prognosis of ALL

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