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Molecular marker and application of grape anthracnose resistance main effect QTL (quantitative trait locus)

A molecular marker and anti-anthracnose technology, which is applied in the detection/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., to achieve the effects of speeding up the progress, shortening the breeding process, and good application value

Inactive Publication Date: 2016-11-30
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of molecular marker and application of grape anti-anthracnose main QTL, thereby solving the aforementioned problems existing in the prior art

Method used

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  • Molecular marker and application of grape anthracnose resistance main effect QTL (quantitative trait locus)
  • Molecular marker and application of grape anthracnose resistance main effect QTL (quantitative trait locus)
  • Molecular marker and application of grape anthracnose resistance main effect QTL (quantitative trait locus)

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0051] Specific Example 1: Discovery of the main QTL for grape resistance to anthracnose and the molecular markers linked to the main QTL.

[0052] 1) Using the anthracnose-resistant wild thorny grape and the anthracnose-sensitive Eurasian species Rizamat to carry out distant hybridization, 203 hybrid populations were obtained.

[0053] 2) Using SSR molecular markers, the authenticity of the hybrid progeny between Grape thorns and Rizamat was identified. The primers that can amplify the specific bands of the father were screened out, and the hybrid offspring were identified. 92 offspring had the specific bands of the father. Combining with field morphological analysis, it was confirmed that they were true hybrids. The identified euhybrids can be used in the next step to construct molecular genetic map and QTL for anthracnose resistance.

[0054] 3) Analyze the two parents and their F1 populations with SRAP and SSR molecular markers, and statistically analyze the genetic types...

specific Embodiment 2

[0069] Specific Example 2: Application of main QTL locus RA and molecular marker M1E12-1500-m for grape resistance to anthracnose in grape breeding

[0070] Using the screened molecular markers linked to the main QTL for grape anthracnose resistance, preliminary detection was carried out on 92 F1 hybrid progenies of 'Black Pearl' and 'Rizamat', in order to detect the molecular markers of this method in grape anthracnose resistance. Practical value in marker-assisted selection. Carry out PCR reaction with the genomic DNA of these 92 offspring and SRAP selective amplification primer combination Me1Em12, the operation process of reaction is with the SRAP molecular labeling method among the embodiment 1, PCR amplification result is in non-denaturing polyacrylamide gel electrophoresis ( The results are shown in the figure), after electrophoresis, determine whether there is a corresponding marker according to the presence or absence of the M1E12-1500-m band, if there is a marker, it...

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Abstract

The invention discloses a molecular marker and application of grape anthracnose resistance main effect QTL (quantitative trait locus), and relates to the field of genetics and breeding of fruit plants. The quantitative trait locus determining grape anthracnose resistance is subjected to QTL positioning for the first time, main-effect QTL locus RA of grape anthracnose resistance is determined, rate of contribution of the locus is 71.5%, and 37.7% of grape anthracnose resistance characteristics can be explained. Accuracy in target trait judgement is improved through the linked molecular marker M1E12-1500-m screened based on the locus, and a foundation is laid for improvement of polygene controlled trait based on molecular breeding. In addition, the molecular marker M1E12-1500-m is linked with the main-effect QTL locus RA at a distance of 0.96cM, and the molecular marker M1E12-1500-m is closely linked with the target locus. Therefore, the obtained marker has good application value, accuracy and efficiency in molecular marker-assisted selection can be improved, and progress of improvement in anthracnose resistance of grapes is accelerated.

Description

technical field [0001] The invention relates to the field of fruit tree genetics and breeding, in particular to a molecular marker and application of a grape anthracnose-resistant main effect QTL. Background technique [0002] Grape plays an important role in my country's fruit tree production. In 2014, my country's grape cultivation area was 767,000 hm2, and its output was more than 12.5 million tons, ranking second and first in the world respectively (FAO, 2014). important economic pillar. With the rapid development of the grape industry, diseases have become the main obstacle to the efficient and high-quality production of grapes. [0003] Grape anthracnose is a major disease that endangers grape production, which can cause 10%-20% reduction in grape yield. Anthracnose causes lesions on fruit grains, which seriously damages grape quality. At present, chemical agents are mostly used to control grape anthracnose, but chemical agents will remain in grape fruit and soil, cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 樊秀彩高晓铭刘崇怀张颖姜建福孙海生李民
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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